Post Translational Addition of Amino Acids - Mechanisms Substrate Recognition

蛋白质降解是各种细胞过程的基础，这些过程包括基因调节、蛋白水解级联的减弱和错误折叠或受损蛋白质的去除。我们正在研究进化保守的降解信号N-degron，它是真细菌和真核生物中N-末端规则降解的信号。N-末端规则途径是一个普遍存在的过程，它针对具有特定N-末端氨基酸的蛋白质进行降解。氨酰-tRNA蛋白转移酶催化底物蛋白质N-末端的不稳定氨基酸的翻译后加成，靶向底物蛋白质的N-末端降解。

Protein degradation is fundamental to a variety of cellular processes which include gene regulation, attenuating of proteolytic cascades and the removal of mis-folded or damaged proteins. We are investigating the evolutionary conserved degradation signal known as the N-degron, which is the signal for N-End Rule degradation in both eubacteria and eukaryotes. The N-end rule pathway is a ubiquitous process that targets proteins for degradation that possess a particular N-terminal amino acid. Aminoacyl-tRNA protein transferases catalyze the post-translational addition of destabilizing amino acids from aminoacyl-tRNAs to the N-termini of substrate proteins, targeting them for N-End rule degradation.