Identification of Giardia species from water samples by PCR and genotyping

莱斯布里奇学院与Hyperion Research Ltd(Hyperion)合作开发了一种快速基因分型方法，用于鉴定呈阳性的贾第虫水样。本项目将利用套式聚合酶链式反应(PCR)、DNA测序和焦磷酸测序技术建立一种简单的分子诊断实验室方法，以区分从贾第虫阳性水样中分离的人致病贾第虫株和非致病株。这些工具将使Hyperion Research Ltd.能够告知他们的客户，如果水样中的贾第虫检测呈阳性，将对公众和人类健康构成风险。该项目还将突出莱斯布里奇学院作为加拿大生物技术创新中心的地位。莱斯布里奇学院还将能够使用通过该项目开发的技术来吸引新的

Lethbridge College has partnered with Hyperion Research Ltd (Hyperion) to develop a rapid genotyping method for characterization of positive Giardia water samples. In this project a simple molecular diagnostic laboratory method will be developed using nested polymerase chain reactions (PCR), DNA sequencing and pyrosequencing techniques to differentiate human pathogenic Giardia strains from non-pathogenic strains isolated from Giardia positive water samples. These tools will give Hyperion Research Ltd. the ability to inform their customers of the public and human health risks of a positive Giardia test from a water sample. This project will also highlight Lethbridge College as a center for biotechnology innovation in Canada. Lethbridge College will also be able to use the techniques developed through this project to attract new