Droplet digital PCR system for new applications in physiology, developmental biology, biomolecular interactions and natural product discovery

资金要求购买新的国家的最先进的系统，可以执行聚合酶链反应，以扩增DNA定量。它被称为液滴数字PCR，是确定组织和细胞中基因表达水平的最敏感和准确的方法。渥太华大学环境基因组学高级研究中心（CAREG）的许多成员研究不同组织中的基因表达或后续处理（例如暴露于环境污染物，激素）。作为一组7个主要用户，我们将使用该机器研究一系列主题，包括脑功能，激素，细菌天然产物，有益真菌和植物药物。因此，本申请的目的是资助一个数字PCR系统，供CAREG的研究团队使用，并培养新的研究生和本科生进行尖端的基因表达分析。不仅他们的研究处于最前沿，他们的培训项目也将得到加强，这将使他们在求职时更具竞争力。

Funds are requested for the purchase of new state-of-the art system that can perform the polymerase chain-reaction to amplify DNA for quantification. It is called droplet digital PCR, and is the most sensitive and accurate way to determine the levels of gene expression in tissues and cells. Many members of the Centre for Advanced Research in Environmental Genomics (CAREG) at the University of Ottawa study gene expression in different tissues or following treatments (e.g. exposure to environmental contaminants, hormones). As a group of 7 major users, we will use the machine to research an array of topics including brain function, hormones, bacterial natural products, beneficial fungi, and phytomedicines. Thus, the purpose of the present application is to fund a digital PCR system for the use of the research teams in CAREG and to train new graduate and undergraduate students in cutting-edge gene expression analysis. Not only will their research be at the cutting-edge, their training program will be enhanced, making them more competitive when seeking employment.