Immobilization, stabilization and biocatalysis of selected enzymatic systems in non-conventional media

申请人研究计划的主要目标是推进和开展实验工作，这些实验工作应有助于理解非常规介质(NCM)，特别是有机溶剂介质(OSM)和无溶剂介质(SFM)中选定的微生物来源的固定化酶的机理作用和生物催化。尽管申请人开展的研究工作涉及脂肪氧合酶/氢过氧化氢裂解酶的固定化和生物催化，但这些酶的稳定性及其在NCM中的作用机理的阐明仍然是需要克服的主要挑战之一。我们实验室在非常规介质中酶的固定化和生物催化方面所获得的知识和经验使我们进入了新的研究领域，特别是使用新的纳米技术方法来固定酶，以及为在非常规介质中阐明其结构和功能而设置实验设计的建模和生物信息学。

The main objective of the applicant's research program is to advance and carry out experimental work that should contribute to the understanding of the mechanistic action and biocatalysis in non-conventional media (NCM), in particular organic solvent media (OSM) and solvent free media (SFM), of selected immobilized enzymes of microbial origin. Although the research work carried out by the applicant addressedthe immobilization and biocatalysis of lipoxygenase/hydroperoxide lyase, the stability of these enzymes as well as the elucidation of their mechanistic action in NCM are still among the major challenges to be overcome. The acquired knowledge and experience in our laboratory in immobilization and biocatalysis of enzymes in non-conventional media led us to move to novel horizons in research, in particular the use of novel nanotechnology approach for enzyme immobilization as well as the modeling and bioinformatics for setting the experimental design for the elucidation of their structure-function in non-conventional media.