Determinating the role of miRNA in myogenic progenitor maintenance and differentiation

这项应用是为了支持我的研究项目，即控制肌肉前体细胞分化的细胞机制。许多胚胎发育事件，包括干细胞和祖细胞的维持，都是通过称为miRNAs的小的非翻译RNA分子的表达在转录后调节的。然而，我们对miRNA如何对单个组织的维持和分化做出贡献知之甚少。我的实验室已经表征了Ars2，我们和其他人已经证明它对于miRNA的生物发生是必不可少的。使用体外C2C12细胞分化培养模型，我的实验室已经证明Ars2是沉默肌源性前体细胞自我更新所必需的，而缺乏Ars2的肌源性细胞缺乏成熟的miRNA。此外，我们已经确定了ARS2功能的潜在调节因子，称为RNPS1，它将Ars2隔离在成肌细胞的细胞质中，直到分化开始。

This application is to support my research program on the cellular mechanisms controlling differentiation of muscle progenitor cells. Many embryonic developmental events, including maintenance of stem and progenitor cells, are regulated post-transcriptionally through expression of small non-translated RNA molecules, called miRNAs. However, we know very little about how miRNA contribute to the maintenance and differentiation of individual tissues. My lab has characterized Ars2, and we and others have shown that it is essential for miRNA biogenesis. Using an in vitro C2C12 cell culture model of differentiation, my lab has shown that Ars2 is necessary for the silencing of self renewal in myogenic progenitor cells, and that myogenic cells deficient for Ars2 are deficient in mature miRNA. In addition, we have identified potential regulator of Ars2 function, called RNPS1, which sequesters Ars2 in the cytoplasm of myoblasts until initiation of differentiation.