Post Translational Addition of Amino Acids - Mechanisms Substrate Recognition

蛋白质降解是多种细胞过程的基础，包括基因调控、蛋白水解级联的减弱和错误折叠或受损蛋白质的去除。 我们正在研究被称为N-degron的进化保守降解信号，这是真细菌和真核生物中N末端规则降解的信号。N-末端规则途径是一种普遍存在的过程，其靶向具有特定N-末端氨基酸的蛋白质进行降解。 氨酰-tRNA蛋白转移酶催化来自氨酰-tRNA的不稳定氨基酸翻译后添加到底物蛋白的N-末端，靶向它们进行N-末端规则降解。

Protein degradation is fundamental to a variety of cellular processes which include gene regulation, attenuating of proteolytic cascades and the removal of mis-folded or damaged proteins. We are investigating the evolutionary conserved degradation signal known as the N-degron, which is the signal for N-End Rule degradation in both eubacteria and eukaryotes. The N-end rule pathway is a ubiquitous process that targets proteins for degradation that possess a particular N-terminal amino acid. Aminoacyl-tRNA protein transferases catalyze the post-translational addition of destabilizing amino acids from aminoacyl-tRNAs to the N-termini of substrate proteins, targeting them for N-End rule degradation.