Development of novel assays for the study of peptide-protein interactions using surface plasmon resonance biosensors

本项目旨在解决为Nicoya生命科学公司基于表面等离子体共振的相互作用分析系统开发新的多肽-蛋白质相互作用分析方案的具体问题。Nicoya有兴趣为研究市场扩展其系统在生物分子相互作用的热力学和动力学分析方面的应用，而Dieckmann实验室在使用基于表面的方法研究特定的蛋白质-多肽相互作用方面经验丰富。该项目利用基于一氧化氮合酶钙调素结合区的蛋白质钙调蛋白与多肽之间的良好相互作用来开发多肽的最佳固定化程序，开发允许测量动力学和热力学参数以及优化和测量传感器性能的分析方法。该项目的成功完成将使尼科亚和加拿大在扩大这一新型传感器平台在研究市场上的应用方面处于有利地位。

This project is intended to solve the specific problem of developing novel peptide-protein interaction assay protocols for the surface plasmon resonance based interaction analysis system from Nicoya Lifesciences. Nicoya is interested in expanding the applications of their system to the thermodynamic and kinetic analysis of biomolecular interactions for the research market and the Dieckmann laboratory is experienced in using surface based methods for the study of specific protein-peptide interactions. This project utilizes the well characterized interactions between the protein Calmodulin and peptides based on the Calmodulin binding region of nitric oxide synthase to develop optimal procedures for the immobilization of the peptides, to develop assays that allow the measurement of kinetic and thermodynamic parameters and to optimize and measure sensor performance. Successful completion of this project will put Nicoya and Canada in a strong position to expand applications of this novel sensor platform into the research market.