Basic studies on biocompatible polymers

生物相容性高分子的基础研究 已经发现用聚合物“刷子”涂覆合成材料可以抵抗但不能消除当未受保护的异物接触血液或组织时发生的排斥反应。我们提出了一些聚合物系统的基础研究，我们正在使用（a）构建更多的生物相容性血袋和（B），以取代人血清白蛋白（HSA）或提供药物与超支化聚缩水甘油（HPG），共享许多HSA的属性。聚合物刷：刷状物是指可溶性聚合物以足够高的密度从其生长的表面，所述密度使得可溶性聚合物被迫形成异常延伸的高能结构。我们已经发现，当任何材料暴露于血液或蛋白质的复杂混合物时，它们可以强烈地影响蛋白质吸附的程度和类型，在特定的刷密度下不同程度地排斥许多蛋白质种类。我们计划对聚苯乙烯乳胶颗粒和扁平聚氯乙烯或二氧化硅芯片上生长的刷子进行基础研究，以更好地了解这些相互作用。具体来说，我们将使用AFM与“胶体”的提示，以研究刷/蛋白质系统的属性;比较这些接触角的研究，使用相分离的水两相聚合物混合物，一个参数，是非常敏感的表面上的大分子结构;使用新的UBC TOF-西姆斯仪器研究暴露于蛋白质混合物的刷子上的混合蛋白质膜的组成，并了解曲率半径的作用刷行为。 HPG：超支化聚合物由于其紧凑的溶液构型而有点像蛋白质，并且可以通过许多可用的OH基团容易地衍生化。疏水结合对于HSA模拟物和药物递送是至关重要的;这种反应的详细研究将作为含有HPGs的混合物的结构性质的函数以及热力学研究来进行，以检查它们与水、其他聚合物和蛋白质的相互作用。 使用各种HPG衍生物，我们将确定第二种大分子物质进入HPG分子内部的途径以及如何控制它。

Basic Studies on Biocompatible Polymers Coating synthetic materials with polymer 'brushes' has been found to resist but not eliminate rejection reactions that occur when unprotected foreign material contacts blood or tissues. We propose basic studies on some polymer systems we are using to (a) construct more biocompatible blood bags and (b) to replace human serum albumin (HSA) or deliver drugs with hyperbranched polyglycidol (HPG) that shares many of HSA's properties. POLYMER BRUSHES: Brushes refer to surfaces from which soluble polymers are grown at sufficiently high density that they are forced into an unusually extended, high energy structure. We have found that they can strongly affect the degree and type of protein adsorption that occurs when any material is exposed to blood or complex mixtures of proteins, rejecting to varying degrees many protein species at particular brush densities. We plan basic studies on brushes grown on both polystyrene latex particles and on flat polyvinyl chloride or silica chips to better understand these interactions. Specifically we will use AFM with 'colloidal' tips to study the properties of brush/protein systems; compare these to contact angle studies using phase separated aqueous two phase polymer mixtures, a parameter that is extremely sensitive to macromolecular structures on surfaces; utilize the new UBC TOF-SIMS instrument to study the composition of mixed protein films on brushes exposed to protein mixtures and understand the role of radius of curvature on brush behavior. HPGs: Hyperbranched polymers act somewhat like proteins because of their compact solution configuration and can be easily derivatized via the many OH groups available. Hydrophobic binding is critical for an HSA mimic and for drug delivery; detailed studies of this kind of reaction will be made as a function of structural properties as well as a thermodynamic study of mixtures containing HPGs to examine their interactions with water, other polymers and proteins. Using various HPG derivatives we will determine the access a second macromolecular species has to the interior of an HPG molecule and how to control it.