Development of a photothermal non-invasive in-vehicle alcohol detection biosensor

中红外(MIR)双波长激光生物传感器的开发和应用(称为波长调制差分光热辐射测量，WM-DPTR)是一种快速、非侵入性的全光学血液酒精诊断监测器，用于加拿大乃至世界各地，与我们的工业合作伙伴酒精对策系统(ACS)合作。我们之前使用皮肤-葡萄糖-血清模体扩散到皮肤的研究表明，在迄今为止的非侵入性技术中，WM-DPTR对葡萄糖的敏感度最高。具有相似皮肤-乙醇-血清模体结构的WM-DPTR的初步测试表明，在乙醇的情况下，应该可以预期类似的性能。项目目标是开发一种车载WM-DPTR酒精互锁系统的实验室原型。该CRD方案的一个主要部分是将生物传感器中的商用双量子级联激光器(QCL)模块替换为单激光器模块，从而在商业级(~500)数量上大幅降低价格。ACS还与滑铁卢大学合作开发廉价(约500美元)和紧凑的中红外激光器，以支持WM-DPTR技术的商业化。 拟议研究的具体目标是：A)对即将组装的生物传感器对水和血清溶液中存在的乙醇以及在模拟人体血液酒精浓度(0-200 mg/dl)的范围内对人体皮肤样本中扩散的乙醇的敏感性和特异性进行详细的定量研究；B)通过将两个QCL激光器替换为一个来简化传感器的设计；以及C)在人体志愿者身上测试该生物传感器。拟议的便携式预原型系统的尺寸将是：一个手持式探头6“x2”x2“，包括激光头、和平号探测器和光学装置；以及车辆仪表盘下的一个6”x6“x2”电子控制单元(ECU)。这些尺寸与当前的ACS车载电化学传感器联锁兼容。

The development and application of a mid-infrared (MIR) two-wavelength laser-based biosensor (named Wavelength-Modulated Differential Photothermal Radiometry, WM-DPTR) is proposed as a fast, noninvasive all optical blood alcohol diagnostic monitor for use in the fight against drunk driving in Canada and potentially world-wide, in collaboration with our industrial partner, Alcohol Countermeasure Systems (ACS), Etobicoke, ON. Our previous research using a skin-glucose-blood serum phantom diffused into skin demonstrated that WM-DPTR has the highest sensitivity to glucose amongst noninvasive techniques to-date. Preliminary testing of WM-DPTR with a similar skin-ethanol-blood serum phantom configuration showed that similar performance should be expected in the case of ethanol. Project goal is to develop a laboratory prototype of an in-vehicle WM-DPTR alcohol interlock system. A major part of this CRD Proposal is to replace the commercial two-quantum cascade laser (QCL)-module in the biosensor with one-laser-module for substantial price decrease at commercial-level (~ 500) quantities. ACS is also collaborating with the University of Waterloo for the development of inexpensive (~$500) and compact mid-IR lasers for supporting the commercialization of WM-DPTR technology. Specific goals of the proposed research are A) to perform detailed quantitative studies of the sensitivity and specificity of the biosensor-to-be-assembled to the presence of ethyl alcohol in water and blood serum solutions, as well as with ethanol diffused in human skin samples simulating interstitial fluid detection, in the range of human blood alcohol concentration (0 - 200 mg/dl); B) to simplify sensor design by replacing two QCL lasers with one; and C) to test the biosensor on human volunteers. The dimensions of the proposed portable pre-prototype system will be: a handheld probe 6"x2"x2" which includes the laser head, the MIR detector and optics; and an electrical control unit (ECU) 6"x6"x2" under the vehicle dashboard. These dimensions are compatible with current ACS in-vehicle electrochemical sensor interlocks.