Using microbial diversity to better inform best management practices at EVRAZ Regina's hydrocarbon waste remediation facility

生物修复利用自然发生的过程来处理有害的废物，并且可以是一种 有效、成本更低、更环保的废物处理机制。最重要的 污染物的分解过程是由微生物介导的生物降解过程 有机体，尤其是细菌。对这些微生物群落的充分了解对于 优化这些设施的运行。拟议的Engage拨款将建立一个合作研究 里贾纳大学生物系与领先钢铁制造商之间的合作 Evraz Inc.北美(以下简称Evraz)。埃夫拉兹·雷吉纳经营着一个“土地农场”， 自1994年以来，允许碳氢化合物废物的系统应用和随后的细分。土质 废物应用后几年的采样表明，在最初的几个月里，有毒碳氢化合物 厘米的土壤退化，这在很大程度上是由于埃夫拉兹在激励和管理方面的勤奋 通过施肥、曝气(通过耕作)和pH控制的细菌过程。然而，自然和 陆地农场中生物修复细菌群落的能力尚不清楚，我们正在合作 建立1.哪些细菌种类组成了栖息在表面的生物修复群落 土地农场和2.细菌群落组成如何对耕作等管理方法作出反应 那受精呢？我们还将研究社区如何在空间上分布在整个场和 在有积水存在的情况下，群落的变化。我们在鉴定细菌方面所做的工作 土地农田土壤可以更好地为这些作业的管理提供信息，并不仅允许开发 不仅是为了更好地向潜在客户推销其业务，也是为了获得最佳实践。

Bioremediation exploits naturally occurring processes to dispose of harmful waste products and can be an effective, less costly, and more environmentally friendly mechanism of waste disposal. The most important processes in the breakdown of contaminants are biodegradative processes that are mediated by microbial organisms, most notably bacteria. An adequate understanding of these microbial communities is important for optimizing functioning of these facilities. The proposed ENGAGE grant will establish a collaborative research partnership between the Department of Biology at the University of Regina and leading steel manufacturer EVRAZ Inc. North America (hereafter EVRAZ). EVRAZ Regina has maintained a "landfarm" which has allowed for systematic application, and subsequent breakdown, of hydrocarbon wastes since 1994. Soil sampling over a number of years post application of waste has shown that toxic hydrocarbons in the first few centimeters of soil are degraded, in a large part due to EVRAZ's diligence in stimulating and managing bacterial processes by fertilizing, aerating (through tilling), and pH control. However, the nature and capabilities of the bioremediating bacterial communities in the landfarm are unknown, and we are partnering to establish 1. which bacterial species make up the bioremediating communities that inhabit the surface of the landfarm and 2. how does bacterial community composition respond to management approaches such as tilling and fertilization? We will also examine how communities are distributed spatially across the farm and the changes in the community in the presence of standing water. Our work in identifying the organisms in land-farm soils can better inform the management of these operations and allow for not only the development of best practices but also for better marketing of its operation to potential customers.