Improving biopolymer yield in the feedstock plant Camelina sativa through hybridization

塑料的制造对环境健康有负面影响。更替的 以生物质为化学中间体和可回收生物聚合物来源的石油原料 是解决这个问题的一种方法。基因转化十字花科植物的研究 富营养性产碱菌使植物能够合成具有 通常从石油中提取的可生物降解塑料的特性。要实现这些产品的高产量 化合物，它们必须在杂交种子中产生，因为当它们存在于营养组织中时，它们会导致贫瘠的植物 成长。因此，授粉控制和杂交是开发植物性材料的主要要求 用于生物塑料生产的原料。我们建议开发一种新的工具--“莱文沃西亚自交不亲和性” (LSI)，用于目前缺乏这种能力的十字花科饲料物种的授粉控制(例如， 山茶花)。这种模式工厂是加拿大最有前途的可再生原料之一，并已被 这是最近一项耗资450万美元的草原基因组测序工作的主题。这座工厂正在开发用于 生物聚合物由加拿大公司Metabolix油籽(萨斯卡通)生产。由基因决定的 将近缘植物阿拉巴米亚的SI基因转化到该物种，我们建议 更好地了解茶树LSI基因的表达和活性，这是 生物聚合物生产过程中实施杂交的有效制度 合作伙伴，Metabolix油籽公司。到目前为止，该公司一直无法在卡梅利纳解决这个问题。 发展对这一新的和有希望的潜在分子生物学的更好的理解 杂交系统将使我们能够促进基础知识的进步，这将 促进向可持续和可生物降解的石油化工替代品来源的过渡 绿色经济。

The manufacture of plastics has negative consequences for environmental health. The replacement of petroleum-based feedstocks with biomass as the source of chemical intermediates and recyclable biopolymers is one solution to this problem. Transformation of plants in the Brassicaceae family with genes from the bacterial species Alcaligenes eutrophus enables plants to synthesize complex organic compounds that possess the properties of biodegradable plastics normally derived from petroleum. To achieve high yields of these compounds, they must be produced in hybrid seed, as when present in vegetative tissue, they lead to poor plant growth. Pollination control, and hybridization is thus a major requirement for exploiting plant-based feedstocks for bioplastic production. We propose to develop a new tool, "Leavenworthia self-incompatibility" (LSI), for pollination control in Brassicaceae feedstock species where this capability is presently absent (e.g., Camelina sativa). This model plant is one of the most promising Canadian renewable feedstocks, and has been the subject of a recent $4.5 M Genome Prairie sequencing effort. This plant is undergoing development for biopolymer production by the Canadian company, Metabolix Oilseeds (Saskatoon). By genetically transforming this species with SI genes from the related plant species, Leavenworthia alabamica, we propose to gain improved understanding of the expression and activity of LSI genes in Camelina, as required for an effective system of enforcement of hybridization in the biopolymer production process developed by our partner, Metabolix Oilseeds. The company has thus far been unable to solve this problem in Camelina. Developing an improved understanding of the underlying molecular biology of this new and promising hybridization system will allow us to contribute to the advancement of fundamental knowledge that will facilitate the transition to sustainable and biodegradable sources of petrochemical substitutes for Canada's green economy.