The Evolution of Genetic Systems in Plants

植物遗传系统的进化

• 批准号: RGPIN-2017-04494
• 负责人: Schoen, Daniel
• 金额: $ 4.23万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=711076
• 关键词: Evolution; Genetic; Systems; Plants

The organization and origin of genetic variation, together with its method of transmission from the parental generation to progeny generations (the “genetic system”) is central to understanding the evolutionary process and to agricultural improvement. Research in the Schoen lab focuses on genetic systems of both wild and cultivated plant species, with emphasis on the evolution of the mechanisms by which inbreeding is prevented and genetic diversity is maintained. We are particularly interested in the evolution of self-incompatibility (SI) and its loss, leading to self-compatibility (SC). The evolution and loss of SI provides a framework in which to examine recurrent patterns and processes in evolution. Coupled with this investigation, we are examining how epigenetic variation arises in populations, how it is maintained, and the fitness consequences of epigenetic variation. We will continue work on the evolution and loss of SI by conducting research directed at examining how inbreeding depression (the major barrier to the evolutionary loss of SI, and a major determinant of crop yield), is purged. This work will be coupled with genomic level analyses of the architecture of inbreeding depression that will provide information on the number and dominance level of loci underlying inbreeding depression. Related to this, we will conduct studies to determine whether newly evolved S-locus genes can function within the context of existing (non-SI related) plant signaling pathways to give rise to the SI phenotype, and thus help explain how this complex phenotype is assembled from individual components. Recent findings suggest that a complete understanding of the role of genetic systems in evolutionary change must now include epigenetic modification. To improve our understanding of the evolutionary significance of epigenetic variation, we will build upon approaches used in our research on genetic systems, namely the application of both theoretical models and experiments. Population genetic modelling of the evolution of epigenetic modification will be aimed at understanding the factors that promote the evolution of this system in plants and help explain the apparent prevalence in plants (compared to metazoans) of transgenerational inheritance of epigenetic variation. Experimental studies will employ population genomic approaches and environmental manipulation aimed at examining how epigenetic modification is induced and inherited, tests for whether it contributes to fitness, and whole genome investigations to scan for genomic signatures of past selection of epigenetic variation. The work proposed here will lead to the training of 3 Ph.D. students, 2 M.Sc. students, a Research Associate, and 6 undergraduate research assistants, and will complement, in part, our applied research aimed at using SI as a breeding tool (and for which we are separately funded).

遗传变异的组织和起源，以及它从亲代到子代的传递方式（“遗传系统”），对于理解进化过程和农业改良至关重要。Schoen实验室的研究重点是野生和栽培植物物种的遗传系统，重点是防止近亲繁殖和保持遗传多样性的机制的进化。 我们特别感兴趣的是自交不亲和性（SI）的进化及其丧失，导致自交亲和性（SC）。SI的演变和丧失提供了一个框架，在其中检查经常性的模式和过程中的演变。 再加上这项调查，我们正在研究表观遗传变异如何在人群中出现，它是如何保持的，以及表观遗传变异的适应性后果。 我们将继续研究SI的进化和丧失，通过研究如何清除近交衰退（SI进化丧失的主要障碍，以及作物产量的主要决定因素）。这项工作将与基因组水平的分析，将提供信息的数量和显性水平的基因座的近亲繁殖抑郁症的架构。 与此相关，我们将进行研究，以确定新进化的S-位点基因是否可以在现有的（非SI相关）植物信号通路的背景下发挥作用，从而产生SI表型，从而帮助解释这种复杂的表型是如何从单个组件组装。 最近的研究结果表明，对遗传系统在进化变化中的作用的完整理解现在必须包括表观遗传修饰。为了提高我们对表观遗传变异的进化意义的理解，我们将建立在我们对遗传系统的研究中使用的方法，即理论模型和实验的应用。表观遗传修饰进化的群体遗传学建模旨在了解促进植物中该系统进化的因素，并有助于解释表观遗传变异跨代遗传在植物中的明显流行（与后生动物相比）。实验研究将采用群体基因组方法和环境操作，旨在研究表观遗传修饰是如何诱导和遗传的，测试它是否有助于适应性，以及全基因组研究，以扫描过去选择表观遗传变异的基因组特征。 这里提出的工作将导致3博士的培训。学生，2个硕士生，一个研究助理，和6个本科研究助理，并将补充，部分，我们的应用研究，旨在使用SI作为育种工具（我们单独资助）。