Controlled Atmospheric Environment for Cell Growth and Manipulation

地球上的许多生物生活在微氧或厌氧环境中，例如在人类的肠道中，它们暴露在决定细胞新陈代谢的各种气体中。因此，在受控和恒定的环境中生长和操纵细胞培养的能力对于理解这些系统的作用机制是必不可少的。Zamble实验室需要受控环境条件来研究几种人类病原体中独特的金属代谢，如幽门螺杆菌和大肠杆菌。尼茨实验室正在开发基于碲的探测器，以研究肿瘤组织中的缺氧，这是另一个氧气可获得性降低的系统，并将独特的标记添加到蛋白质上。所需的设备提供了所需的各种气体环境，并支持培养、操作和分析，突出了其作为我们研究计划核心基础设施的中心地位。我们目前没有这样的能力。 加在一起，在我们的团队中，我们目前正在指导15名学生和博士后研究员，所有这些受训人员都将接受这一关键基础设施的培训，并可以使用这些基础设施。此外，这种类型的仪器通常用于生物技术、生物和制药行业，因此动手培训和方法开发对学生在这些领域未来的就业机会是有益的。

Many organisms on this planet live under micro-aerobic or anaerobic environments, such as in the human gut, where they are exposed to a variety gases that dictate cellular metabolism. As such, the ability to grow and manipulate cell cultures under a controlled and constant environment is essential to understanding the mechanisms of action of these systems. The Zamble lab requires controlled-environment conditions to study the unique metal metabolism in several human pathogens such as Helicobacter pylori and Escherichia coli. The Nitz lab is developing tellurium based probes to study hypoxia in tumor tissue, another system with reduced oxygen availability, and to put unique labels into proteins. The equipment requested delivers the breadth of gaseous environments required, and supports culture growth, manipulation, and analysis, highlighting its central position as core infrastructure for our research programs. We currently do not have such capabilities. Combined, in our groups we are currently mentoring 15 students and post-doctoral fellows, and all of these trainees will be trained on, and have access to, this key piece of infrastructure. Furthermore, this type of instrument is commonly used in the biotechnology, biological, and pharmaceutical industries, so hands-on training and method development is beneficial to students seeking future employment in these areas.