Lentivirus Rev protein: characterization and study of Rev - nucleolar protein interactions

定义宿主细胞和病毒蛋白之间的相互作用对于理解病毒如何利用细胞功能复制和规避控制病毒复制的细胞先天策略至关重要。其中重要的逆转录病毒蛋白是慢病毒Rev。Rev蛋白定位于受感染细胞的细胞核和核仁。Rev的主要功能是将未剪接和单剪接的病毒转录本从细胞核转运到细胞质，以完成复制。然而，人类免疫缺陷病毒1型（HIV-1）Rev最近被重新审视，其他功能，如调节细胞基因组内的病毒基因组整合和参与病毒组装已被证明为Rev。此外，HIV-1 Rev被证明与大量的细胞蛋白质相互作用，其中大部分是细胞质或细胞核来源。到目前为止，HIV-1和牛免疫缺陷病毒（BIV）的结构域已被研究和表征最多。

Defining interactions between host cells and viral proteins is essential in understanding how viruses exploit cellular functions to replicate and circumvent the cell innate strategies underlying the control of virus replication. Among important retroviral proteins is the lentiviral Rev. The Rev protein localizes to the nuclei and nucleoli of infected cells. Rev main function is to transport from the nucleus to the cytoplasm the unspliced and singly spliced viral transcripts to fullfil replication. However, the Human immunodeficiency virus type 1 (HIV-1) Rev was recently revisited and other functions such as regulating viral genome integration within the cell genome and participating in virus assembly have been demonstrated for Rev. In addition, HIV-1 Rev was shown to interact with a significant number of cellular proteins the majority of which were of cytoplasmic or nucleus origin. So far structural domains of HIV-1 and the Bovine immunodeficiency virus (BIV) have been the most studied and characterized.