Quantitative microfluidic delivery systems for studying tissue microenvironment and microvascular blood flow regulation in vivo

所有身体组织的营养需求都是由血流到全身微血管网络的动态分布提供的。研究体内的血流调节需要专门的、微创的手术准备，以便分离感兴趣的组织，同时保持调节系统本身的正常功能和控制。我们之前已经开发了气体交换室来操纵活鼠骨骼肌中的气体分压。这项工作证明了这些装置的可行性和有效性，并且微循环可以响应周围微环境的变化。

The nutritive requirements of all body tissues are provided for by the dynamic distribution of blood flow to microvascular networks throughout the body. Studying blood flow regulation in vivo requires specialized, minimally invasive, surgical preparations in order to isolate a tissue of interest while preserving the normal function and control of the regulatory system itself. We have previously developed gas exchange chambers to manipulate gas partial pressures in skeletal muscle of live rats. This work demonstrated the feasibility and efficacy of these devices and that the microcirculation can respond to changes in the surrounding microenvironment.