Cryopreservation of livestock colostrum-derived mesenchymal stem cells

目前，抗生素被广泛用于预防和控制猪和牛等农场动物的疾病和感染。然而，在供人类食用的动物中继续使用抗生素可能会导致对抗生素产生抗药性的细菌的生长。加拿大卫生部正在采取行动，逐步停止在畜牧业中使用某些抗生素，以限制肉类和其他动物产品中抗药性细菌对公众构成的危险。因此，需要新的、安全和有效的策略，通过在不使用抗生素的情况下预防疾病/感染来提高牲畜的整体免疫力和总体健康。MyStem Biotech Inc.(MySteMBiotech)正在开发一种新产品，该产品由猪和牛的奶中的细胞组成，可以用来代替抗生素。细胞的来源是初乳，这是动物在分娩后的几天内释放的“第一乳”。虽然众所周知，初乳中含有丰富的抗体，但他们的初步结果表明，初乳中也含有一种特殊类型的细胞，称为间充质干细胞(MSCs)。这些细胞能够增强人体对可能导致疾病的微生物的保护反应。该公司已经成功地从猪和牛的初乳中分离出这些细胞，并在培养中培养它们，以增加它们的数量。我们在活细胞和组织长期存储领域的专业知识将满足公司对冷冻细胞的策略的需求，以便它们可以保持活着以供未来使用，并以一种可以方便和安全地提供给农场动物的形式。这项研究预计将产生的使能和创新技术代表了一种自然和经济的方法，以提高加拿大牲畜的健康，并促进农业和农业食品业。

Currently, antibiotics are widely used to prevent and control disease and infections in farm animals such as pigsand cows. However, the continued use of antibiotics in animals meant for human consumption can cause thegrowth of bacteria that become resistant to antibiotics. These harmful bacteria can also be passed on to humansvia the food chain. Health Canada is moving to phase out the use of some antibiotics in the animal industry tolimit the danger posed to the public by antibiotic-resistant bacteria in meat and other animal products.Therefore, new, safe and efficient strategies are needed to improve overall immunity and general health oflivestock by preventing disease/infection without the use of antibiotics. MySteM Biotechnologies Inc.(MySteMBiotech) is developing a new product composed of cells from the milk of pigs and cows that can beused instead of antibiotics. The source of the cells is the colostrum, the "first milk" released during the fewdays within the time the animals give birth. While it is widely known that colostrum is rich in antibodies, theirpreliminary results show that it also contains a special type of cells called mesenchymal stem cells (MSCs).These cells have the ability to boost the body's protective response against microorganisms that can causedisease. The company has successfully isolated these cells from pig and cow colostrum and grown them inculture to increase their numbers. They have also shown that these cells have the ability to wipe out bacteria inculture. Our expertise in the field of long-term storage of live cells and tissues will address the company's needfor strategies to freeze the cells so they can be kept alive for future use and in a form that can be readily andsafely given to farm animals. The enabling and innovative technology that is anticipated to result from thisstudy represents a natural and economic approach to enhance the health of Canada's livestock and boost ouragriculture and agri-food industry.