Nanoporous Media in Analytical Sciences

该方案提出利用微流控技术，以提高生化分析和临床诊断在一个领域的应用。另一方面，我们将更深入地了解纳米多孔介质中的输运，以便更好地模拟裂缝固体介质（岩石）中的水、油和天然气。该项目将改进蛋白质的分离方法，开发离心微流体装置和工程表面，以改善代谢物的临床诊断。它还将提供纳米多孔介质输运的基本数据，这将有助于建模和理解裂缝岩石中的输运，从而有助于提高油气采收率。******纳米多孔材料的开发和利用是本工作中所追求的每个应用的中心主题。纳米多孔材料可以合理设计，以提高材料在化学和生化分析中的应用。在我们的项目中，我们利用自组装和制造方法来制造纳米多孔设备和表面，并在生命科学中应用。我们的重点是蛋白质分离，在肽，蛋白质和代谢物质谱。制造技术使我们能够设计纳米多孔介质的孔径、顺序和材料特性，并结合表面化学控制材料的特性和性能。我们将能够提高分析性能，或创造新的分析手段，使用我们制造纳米多孔材料的方法。******在纳米多孔材料的不同应用中，我们将利用我们的方法所允许的孔隙度的精细控制，来更深入地了解纳米多孔介质中的传输。在破裂的岩石介质中，存在大小尺度的组合，但由于纳米孔的流体力学尚未得到很好的理解，因此在预测整体运移和液体回收结果方面，建立数学模型变得更具挑战性。这项工作将提供实验结果，通过实验测量最小孔隙尺寸的参数，可以改进混合尺寸介质（如多孔岩石）中运移和采收率的建模

The program proposed utilizes microfluidic technologies to enhance biochemical analysis and clinical diagnostics in one area of application. In another, we will provide a deeper understanding of transport in nanoporous media for better modeling of water, oil and natural gas in fractured solid media (rocks). The program will improve separation methods for proteins, develop centrifugal microfluidic devices and engineered surfaces for improved clinical diagnostics of metabolites. It will also provide fundamental data on transport in nanoporous media that will be of benefit in the modeling and understanding of transport in fractured rock, so as to aid in enhanced oil and gas recovery.******The development and exploitation of nanoporous materials is the central theme to each of the applications pursued in this work. Nanoporous materials can be rationally designed to enhance the desired properties of a material for applications in chemical and biochemical analysis. In our program we are utilizing both a self-assembled and a manufacturing methodology to create nanoporous devices, and surfaces, with applications in the life sciences. Our focus is on protein separation, and in peptide, protein and metabolite mass spectrometry. The fabrication techniques allow us to engineer the pore size, order and material properties of the nanoporous media, which, combined with surface chemistry controls the properties and performance of the materials. We will be able to improve analytical performance, or create new means of analysis, using our methods of fabrication of nanoporous materials. ******In a different application of nanoporous materials, we will utilize the fine control of porosity that our methods allow, to provide a deeper understanding of transport in nanoporous media. In fractured rock media a combination of large and small scale regimes exist, but since the fluid mechanics of nanopores are not well understood they become more challenging to model mathematically in terms of predicting overall transport and liquid recovery outcomes. This work will provide experimental results that allows improved modeling of transport and recovery in mixed size scale medium such as porous rock, through experimentally measuring the parameters of the smallest pore sizes.**