Mass Transfer Coefficient Measurement in Single-use Bioreactor - Application of the Endoscopy Technique

提供规模更大、成本更低的疫苗有着巨大的需求。目前，经济上**优化的解决方案似乎采用了更大规模的一次性生物处理系统。这降低了**现有疫苗的生产成本，并最终促进了新疫苗的开发。**赛诺菲巴斯德拥有最广泛的疫苗种类，保护人们免受26种**传染病中的20种感染。加拿大最大的疫苗生产公司之一赛诺菲的疫苗部门**对利用一次性充气搅拌生物反应器在工业规模生产疫苗方面感兴趣。**尽管充气搅拌生物反应器普遍使用，但关于各种工艺变量**对一次性生物处理系统性能的影响的了解仍然有限。许多工业中使用的充气搅拌生物反应器的性能在很大程度上取决于将氧气从气相转移到反应器内的液体的能力。理解氧气传质的一个关键部分是能够确定生物反应器中气泡的大小。因此，**拟议研究项目的目标是研究生物反应器中气/液相互作用的流体动力学，作为设计参数和操作条件的**函数，并开发一种优化设计，以获得**有效的氧气传质速率，明确地说是曝气搅拌生物反应器内生产疫苗所需的体积传质系数。为了实现这些目标，采用了广泛的实验**，包括先进的可视化和图像分析技术，如内窥镜(EnviroCam)和光学**溶解氧仪(DO)。**拟议项目的结果将使加拿大生物加工公司和疫苗**制造商能够在其生产系统中应用一次性生物反应器。这将带来更高的**产品质量、高效的电力使用、更高的生产能力以及资本/运营成本的节约。

There is an enormous demand to provide vaccines at higher scales and lower costs. Currently the economically**optimized solution seems to employ larger size single-use bioprocessing systems. This results in lowering**existing vaccines production cost and ultimately facilitating the development of new vaccines.**Sanofi Pasteur has the broadest available range of vaccines, protecting population against 20 out of 26**infectious diseases. The vaccines division of Sanofi, one of Canada's largest vaccine producing company is**interested in utilizing a single-use aerated stirred bioreactor for the production of vaccines at an industrial scale.**Despite the common use of the aerated stirred bioreactors, knowledge about the influence of various process**variables on performance of the single-use bioprocessing system is still limited. The performance of the aerated**stirred bioreactors utilized in numerous industries is highly dependent on the ability to transfer oxygen from the**gas phase to the liquid phase inside the reactors. A key part of understanding the mass transfer of oxygen is**related to being able to determine the size of the gas bubbles in the bioreactors. Therefore the goals in the**proposed research project are to investigate the hydrodynamics of gas/liquid interaction in the bioreactors as a**function of the design parameters and operating conditions and to develop an optimal design in obtaining an**efficient oxygen mass transfer rate, explicitly the volumetric mass-transfer coefficient, required for the vaccine**production inside of the aerated stirred bioreactor. To achieve these objectives, extensive experimentations**including advanced visualization and image analysis technique such as endoscopy (EnviroCam), and optical**dissolved oxygen meter (DO) are employed.**Results from the proposed project will enable the Canadian bioprocessing companies and vaccine**manufacturers to apply the single-use bioreactors in their production systems. This will lead to an enhanced**product quality, efficient use of power, increased production capacity, and capital/operational cost savings.