Genetic enhancement of cellulose production by Consenzeae myxofaciens

粘菌杆菌科，也被称为粘菌变形杆菌，是一种革兰氏阴性细菌，与mirabilis等属同一科。这种菌株因其生产大量生物纤维素的能力而在工业上具有重要意义。生物纤维素显示出不同于植物基纤维素的特性，特别是它的长纤维长度和独特的成分，使其在生物医学、电子和食品科学领域的应用中特别有用。细菌生物纤维素传统上是由细菌生产的，如**葡萄糖杆菌xylinus，一种绝对需要氧气才能生长的细菌。然而,* * C。黏菌是一种兼性微生物，能够在有或没有氧气的情况下生长，这使得该菌株对工业更有用。我们的实验室已经开始开发基因工具来改造这种生物，最终目标是使这种细菌减少潜在的病原体，增加和更稳定的生物纤维素产量。这些工具包括建立转座子插入文库，以及有效地将外源DNA引入c.m myxofaciens的方法，以便通过特异性地删除它们（无标记删除）来特异性地灭活基因。该提案的目的是利用这些技术创造出更安全的转基因粘菌杆菌菌株，并且能够以更低的成本和更高的产量生产生物纤维素。

Cosenzeae myxofaciens, also referred to as Proteus myxofaciens is a Gram-negative bacterium in the same**family as species such as P.mirabilis. This strain is industrially important for its ability to produce large**quantities of biocellulose. Biocellulose displays properties different from plant-based cellulose, notably its**long fiber length and unique composition, making it particularly useful in applications in the biomedical,**electronic and food science fields. Bacterial biocellulose has been traditionally produced by bacteria such as**Gluconeobactobacter xylinus, a bacterium with an absolute requirement for oxygen in order to grow. However,**C.myxofaciens is a facultative microbe, able to grow in the presence or absence of oxygen, making the strain**much more useful to industry. Our laboratory has begun to develop genetic tools to modify this organism with**the ultimate goal of making the bacterium less of a potential pathogen, with increased and more stable**biocellulose production. These tools include the creation of a library of transposon insertions, and methods of**effectively introducing foreign DNA into C.myxofaciens in order to specifically inactivate genes by specifically**deleting them (markerless deletion). The aim of this proposal is to use these technologies to create genetically**modified C.myxofaciens strains that are safer and are able produce biocellulose at reduced cost and at a greater**yield.