Development of stabilized environmentally sensitive polymeric nanoparticles

研究问题：纳米粒子已经被广泛探索作为载体，将疏水分子运送到目标位置。疏水分子在水中不易分散，容易聚集，从而影响其功效。纳米粒子克服了这一挑战，为疏水有效载荷提供了疏水核心，同时提供了亲水外壳，促进水溶液中的自由分散。纳米颗粒通过封装货物，避免在货物到达目的地之前与不稳定剂的不良接触，从而提高有效载荷的稳定性。最近，在开发环境敏感纳米颗粒方面取得了进展，这些纳米颗粒可以释放其有效载荷以响应环境刺激，这些刺激可以是化学的（pH、氧化还原）或物理的（温度、光）。提高纳米颗粒的稳定性和负载效率是各种应用的关键考虑因素。***建议研究：我的工作旨在开发对环境敏感的合成聚合物纳米颗粒。它们保持稳定，直到它们暴露在以低pH值、高还原电位或温度变化为特征的环境中。这些纳米粒子将有潜在的应用，作为生物活性药物的载体来治疗疾病或作为生物传感器来监测生物过程。***该计划的目标是设计具有环境敏感性的稳定纳米颗粒的生物聚合物。聚合纳米颗粒将被合成具有疏水核和亲水壳。氧化还原、pH值和温度敏感性将被引入聚合物。其基本原理是，纳米颗粒可以被设计成在暴露于一个或几个这些刺激时，以预先编程的方式有效地释放它们的货物。例如，由于细胞内环境中的低pH值和高水平的还原剂，pH和氧化还原敏感性可以促进细胞内货物的递送。此外，温度变化会导致纳米颗粒的形态变化，从而促进货物的释放。本研究还将通过研究不同的交联方法，并比较它们对稳定性和有效载荷释放的影响，来提高纳米颗粒的稳定性。为了研究疏水性对包封的影响，我们将研究不同的疏水分子链，并评估由此产生的负载效率。***预期结果：该研究项目将优化聚合物纳米颗粒作为化学试剂载体的结构和性能，并将推进我们对具有环境敏感性的功能聚合物的认识。这项研究有潜力通过开发丰富的新材料来促进加拿大纳米材料工业的发展。拟议的项目将为不同层次的HQP提供尖端培训

Research Problem: Nanoparticles have been extensively explored as carriers to deliver hydrophobic molecules to targeted locations. Hydrophobic molecules do not disperse easily in water and can aggregate, thereby compromising their efficacy. Nanoparticles overcome this challenge by offering a hydrophobic core for hydrophobic payloads, while providing a hydrophilic shell that promotes free dispersion in aqueous solution. Nanoparticles offer the additional benefit to improve payload stability by encapsulating cargos to avoid adverse contact with destabilizing agents before their destination. Recently, progress has been made in developing environmentally sensitive nanoparticles that release their payloads in response to environmental stimuli, which can be either chemical (pH, redox) or physical (temperature, light). Improving the stability and loading efficiency of nanoparticles is a key consideration for a variety of applications. ***Proposed Research: My work is aimed at developing synthetic polymeric nanoparticles that are environmentally sensitive. They remain stable until they are exposed to an environment characterized by low pH, a high reducing potential or a temperature change. These nanoparticles will have potential applications as carriers of bioactive agents to treat diseases or to monitor biological processes as a biosensor.***The goal of the proposed program is to design biopolymers for stabilized nanoparticles with environmental sensitivities. Polymeric nanoparticles will be synthesized with a hydrophobic core and a hydrophilic shell. Redox, pH, and temperature sensitivities will be introduced into the polymers. The rationale is that the nanoparticles can be designed to efficiently release their cargos upon exposure to one or several of these stimuli in a pre-programmed manner. For example, pH and redox sensitivities can facilitate intracellular delivery of cargos due to the low pH value and high level of reducing agents in the intracellular environment. In addition, temperature change can lead to a morphology change of nanoparticles that can promote cargo release. The proposed research will also improve the stability of nanoparticles by studying different crosslinking methods and comparing their effects on stability and payload release. In order to study the effects of hydrophobicity on encapsulation, we will study different hydrophobic molecular chains and assess the resulting loading efficiency. ***Expected Outcome: The research program will optimize the structure and performance of polymeric nanoparticles as carriers of chemical agents, and will also advance our knowledge of functional polymers with environmental sensitivities. This research has the potential to promote the growth of the Canadian Nanomaterials Industry by developing a wealth of new materials. The proposed program will provide cutting edge training for HQP at different levels.**