Nanoporous Media in Analytical Sciences

提出的计划利用微流控技术在一个应用领域加强生化分析和临床诊断。在另一门课程中，我们将更深入地了解纳米多孔介质中的传输，以便更好地模拟裂隙固体介质(岩石)中的水、石油和天然气。该计划将改进蛋白质的分离方法，开发离心式微流控设备和工程表面，以改善代谢物的临床诊断。它还将提供有关纳米多孔介质中输运的基本数据，这些数据将有助于模拟和理解裂隙岩石中的输运，从而有助于提高石油和天然气的采收率。纳米多孔材料的开发和开发是这项工作所追求的每一种应用的中心主题。纳米多孔材料可以被合理地设计来提高材料在化学和生化分析中的应用所需的性能。在我们的计划中，我们正在利用自组装和制造方法来创造纳米多孔设备和表面，并将其应用于生命科学。我们的重点是蛋白质分离，以及肽、蛋白质和代谢物的质谱学。制造技术使我们能够设计纳米多孔介质的孔大小、有序和材料特性，结合表面化学控制材料的特性和性能。我们将能够改进分析性能，或创造新的分析手段，使用我们制造纳米多孔材料的方法。*在纳米多孔材料的不同应用中，我们将利用我们的方法允许的对孔隙率的精细控制，以提供对纳米多孔介质中传输的更深层次的理解。在破碎的岩石介质中，存在大尺度和小尺度的组合，但由于纳米孔的流体力学还没有被很好地理解，所以在预测总体运移和液体回收结果方面，它们变得更具挑战性。这项工作将提供实验结果，通过实验测量最小孔隙尺寸的参数，改进混合尺寸介质(如多孔岩)中的传输和回收模型。

The program proposed utilizes microfluidic technologies to enhance biochemical analysis and clinical diagnostics in one area of application. In another, we will provide a deeper understanding of transport in nanoporous media for better modeling of water, oil and natural gas in fractured solid media (rocks). The program will improve separation methods for proteins, develop centrifugal microfluidic devices and engineered surfaces for improved clinical diagnostics of metabolites. It will also provide fundamental data on transport in nanoporous media that will be of benefit in the modeling and understanding of transport in fractured rock, so as to aid in enhanced oil and gas recovery.******The development and exploitation of nanoporous materials is the central theme to each of the applications pursued in this work. Nanoporous materials can be rationally designed to enhance the desired properties of a material for applications in chemical and biochemical analysis. In our program we are utilizing both a self-assembled and a manufacturing methodology to create nanoporous devices, and surfaces, with applications in the life sciences. Our focus is on protein separation, and in peptide, protein and metabolite mass spectrometry. The fabrication techniques allow us to engineer the pore size, order and material properties of the nanoporous media, which, combined with surface chemistry controls the properties and performance of the materials. We will be able to improve analytical performance, or create new means of analysis, using our methods of fabrication of nanoporous materials. ******In a different application of nanoporous materials, we will utilize the fine control of porosity that our methods allow, to provide a deeper understanding of transport in nanoporous media. In fractured rock media a combination of large and small scale regimes exist, but since the fluid mechanics of nanopores are not well understood they become more challenging to model mathematically in terms of predicting overall transport and liquid recovery outcomes. This work will provide experimental results that allows improved modeling of transport and recovery in mixed size scale medium such as porous rock, through experimentally measuring the parameters of the smallest pore sizes.**