Replacement of key laboratory infrastructure for measurement of metabolism in aquatic animals

要求一种用于甲壳类动物的Loligo系统光纤多通道间歇呼吸系统。该系统由硬件和软件组成，用于测量从细胞水平到大型甲壳类动物的氧气消耗（MO2）。该资金申请还包括一台松下防水笔记本电脑，专用于该系统。这笔资金申请是为了替换McGaw博士实验室的一个关键基础设施。目前在McGaw实验室有一个较旧的（bbbb10年）电氧系统，它几乎一直被各种培训人员（高素质人员，HQP）使用。然而，它已经到了使用寿命的尽头。该软件不再受支持，系统多次崩溃。探针本身不断地漂移，这使得长期实验不可能进行，而且该系统只能准确地记录较大动物（bbb20克）的MO2。探针本身需要经常注意，并已多次更换。因此，数据的类型和收集准确数据的时间限制了许多可以进行的实验的类型和长度。光纤系统远远优于旧的电系统：它是高度精确的，传感器不需要维护，几乎零漂移，使其适合长期实验。光纤系统可以有很长的电缆长度（例如20米），允许“远程”监控。此外，计算机、冷水机和水泵产生的电气噪声会影响电探针，系统中使用的Witrox仪器与PC有数字无线蓝牙通信，因此模拟数据电缆少得多，因此噪音也少得多。由于传感器具有各种尺寸（<50 μ m）和快速分辨率，因此它将使McGaw博士和他的HQP能够准确地记录各种不同大小生物体的长期MO2。虽然二氧化氧的测量并不新颖，但所收集的数据对于生物体生理学实验至关重要，构成了许多论文的支柱，然后导致HQP的进一步问题和新项目。该设备将用于培训McGaw实验室现在和未来的研究生，并将在不使用时提供给其他研究人员，因此，将有助于研究项目和大量受训人员的HQP经验。它将成为实验室基础设施的一个关键部分，将大量用于各种各样的实验。因此，它还将为HQP提供在学术环境、政府机构、咨询公司和其他私营部门公司中有价值的知识和技能。

A Loligo systems fiber optic, multi-channel intermittent respiratory system for use in crustaceans is requested. The system consists of hardware and software that is used to measure oxygen consumption (MO2) all the way from the cellular level to that of large crustaceans. This funding request also includes a Panasonic waterproof laptop computer to be dedicated to the system. This funding request is to replace a key piece of infrastructure in Dr. McGaw's lab. There is currently an older (>10yr) galvanic oxygen system in the McGaw lab, which has been in use almost constantly by various trainees (Highly Qualified Personnel, HQP). However, it has come to the end of its useful life. The software is no longer supported and the system has crashed on a number of occasions. The probes themselves drift continually which makes longer term experiments impossible and the system is only accurately able to record MO2 in larger animals (>20g). The probes themselves need constant attention and have been replaced on numerous occasions. Thus the type of data and the time to gather accurate data limits the type and length of a lot of the experiments that can be performed. The fibre optic system is far superior to the older galvanic system: it is highly accurate and the sensors do not need maintenance and have almost zero drift which makes it suitable for longer term experiments. The fibre optic system can have long cable lengths (e.g. 20 m) which allows “remote” monitoring. In addition, computers, water chillers and water pumps generate electrical noise that can affect the galvanic probes, the Witrox instruments used in the system have digital wireless Bluetooth communication with the PC, so there are lot less analog data cables and thus a lot less noise. Because the sensors come in a variety of sizes (<50µm) with rapid resolution it will enable Dr. McGaw and his HQP to accurately record long term MO2 over a wide range of different sized organisms. While the measurement of MO2 is not novel, the data gathered are essential for organismal physiology experiments, forming the backbone of many papers, which then lead on to further questions and new projects for HQP. This equipment will be used to train present and future graduate students in the McGaw lab and will be available for other researchers when not in use, and thus, will contribute to the research projects and experience of a large number of trainees HQP. It will form a key piece of laboratory infrastructure that will be heavily used in a wide variety of experiments. As such it will also provide HQP with the knowledge and skills that will be valuable in the academic environment, to government agencies, and to consulting firms and other private sector companies.