Prevention of SARS-CoV2 (COVID-19) related CoV Spike protein neuroinvasion and -induced respiratory distress by polyphenols

在本研究中，我们提出证明多酚可以抑制SARS-CoV-2蛋白之间的相互作用，从而抑制病毒的有害影响。此外，随着SARS-CoV-2侵入神经系统进而影响大脑的证据越来越多，我们建议在呼吸中枢水平研究SARS-CoV-2蛋白的作用以及多酚的预防作用。为此，我们建议采用基于理论（分子建模），实验（DLS， SPR，多酚-蛋白质复合物的实时可视化）方法的多学科方法来更好地了解蛋白质与酚类配体之间的物理化学相互作用。这些理论和实验方法的结果将在脑呼吸中枢背根神经节的生物学模型上完成。首先，将研究SARS-CoV-2蛋白对这些神经节的毒性作用，然后研究这些蛋白与多酚相互作用后的保护作用。这项研究的结果将使我们能够获得关于SARS-CoV-2对呼吸系统问题有害影响的新知识，并证明和鉴定对-à-vis SARS-CoV-2具有保护潜力的多酚。这些结果将为神经侵袭机制的研究和SARS-CoV-2治疗方法的开发开辟多个视角。

In this research, we propose to demonstrate the concept that polyphenols can inhibit interactions between the proteins of SARS-CoV-2 and thus inhibit the harmful effects of the virus. In addition, as the evidence accumulates on the invasion of SARS-CoV-2 in the nervous system and thus affect the brain, we propose to study the effects of the proteins of SARS-CoV-2 at the level of the respiratory center and the preventive effect of polyphenols. To do this, we propose to use a multidisciplinary approach based on theoretical (molecular modeling), experimental (DLS, SPR, real time visualization of polyphenol-protein complexes) methods to better understand the physicochemical interactions between proteins and phenolic ligands. The results of these theoretical and experimental approaches will be completed on biological models, of the ganglia of the dorsal root of the cerebral respiratory center. First, the toxic effects of the proteins of SARS-CoV-2 will be studied on these ganglia, then the protective effects following the interaction of these proteins with polyphenols. The results of this study will allow to acquire new knowledge on the harmful effects of SARS-CoV-2 on respiratory problems, to demonstrate and identify polyphenols having a protective potential vis-à-vis SARS-CoV-2. These results will open multiple perspectives both on the study of neuroinvasive mechanisms and on the development of treatments against SARS-CoV-2.