Manufacturing and scaling of an in vitro human skeletal muscle phenotypic assay (I2I Phase I)

我们已经创建了一个预测性的人类表型测定定量肌肉干细胞介导的骨骼肌修复在一个盘子。这使我们能够识别在健康和患病情况下对肌肉修复产生积极或消极影响的因素和分子。目前，需要扩大表型分析制造和数据分析，并确定分析的可重复性限制了我们的平台从学术环境到工业应用的转化。因此，在这个第一阶段的申请中，我们寻求I2I资金，为我们的平台建立可扩展的制造方法和自动图像分析管道，并量化在规模化平台内获得的结果的可重复性。克服这些工程挑战将使我们能够在安大略省成立一家初创公司，并/或将技术授权给现有的生物技术公司。

We have created a predictive human phenotypic assay to quantify muscle stem cell-mediated skeletal muscle repair in a dish. This enables us to identify factors and molecules that positively or negatively impact muscle repair in the context of healthy and diseased situations. Currently, the need to scale the phenotypic assay manufacturing and data analysis, and to determine assay reproducibility limits the translation of our platform from an academic setting into industrial use. Hence, in this Phase I application we seek I2I funds to establish a scalable manufacturing method and automated image analysis pipeline for our platform, and to quantify the reproducibility of outcomes obtained within the scaled platform. Overcoming these engineering challenges will position us to form an Ontario-based start-up and/or license the technology to an existing biotech company.