Predicting patient outcome using machine learning technique

化学探针是生物成像工具箱的关键组成部分，因为它们标记细胞和组织中的生物分子。生物成像的新挑战是设计用于三维组织成像的化学探针。在这项工作中，我们发现金属纳米颗粒的光散射可以在完整和透明的组织中提供3D成像对比。纳米颗粒可以作为金属层化学生长的模板，进一步增强散射信号。在整个组织中使用化学培养的纳米颗粒可以放大散射，产生比使用普通荧光团高140万倍的光子产量。这些探针是非光漂白的，可以与？无干扰的荧光团。我们展示了三种不同的生物医学应用：(a)血管的分子成像，(b)肿瘤中纳米药物载体的跟踪，以及(c)多发性硬化症小鼠模型中病变和免疫细胞的定位。我们的策略建立了一套独特而又互补的成像探针，用于从三维角度理解疾病机制。

Chemical probes are key components of thebioimaging toolbox, as they label biomolecules in cells andtissues. The new challenge in bioimaging is to design chemicalprobes for three-dimensional (3D) tissue imaging. In thiswork, we discovered that light scattering of metal nanoparticlescan provide 3D imaging contrast in intact and transparenttissues. The nanoparticles can act as a template for thechemical growth of a metal layer to further enhance thescattering signal. The use of chemically grown nanoparticles inwhole tissues can amplify the scattering to produce a 1.4million-fold greater photon yield than obtained using common?uorophores. These probes are non-photobleaching and canbe used alongside ?uorophores without interference. Wedemonstrated three distinct biomedical applications: (a) molecular imaging of blood vessels, (b) tracking of nanodrug carriers intumors, and (c) mapping of lesions and immune cells in a multiple sclerosis mouse model. Our strategy establishes a distinct yetcomplementary set of imaging probes for understanding disease mechanisms in three dimensions.