Isolation and identification of Tenacibaculum bacteria from a Chinook Salmon saltwater production site

腱杆菌存在于世界各地的沿海海洋环境中，包括加拿大。已经证明，在养殖的大西洋鲑鱼上，有几种Tenacibaculum是机会致病菌，然而，对这种细菌的海洋生态和太平洋鲑鱼中Tenacibaculum感染的了解有限。本项目旨在确定在不列颠哥伦比亚省的奇努克鲑鱼咸水生产基地内和周围的腱杆菌细菌。该项目将建立在圭尔夫大学（UGuelph）、温哥华岛大学（VIU）、创意鲑鱼公司有限公司和卑诗省水产健康科学中心（CAHS）之间强有力的合作伙伴关系的基础上。该项目的目的是：1)收集和测试奇努克鲑鱼咸水网圈内及其周围的腱藻物种的水样；2)在咸水生产过程中收集并检测奇努克鲑鱼组织中腱杆菌的种类；3)在海水生产地收集和测试Tenacibaculum物种的生物（无脊椎动物）和非生物（net-pen基础设施）生物和表面；4)利用高通量分子分析来调查所收集样本中Tenacibaculum物种的群落分布和相对丰度。该项目将提供对更广泛的海洋环境中Tenacibaculum物种存在的理解，并有助于我们了解细菌在太平洋鲑鱼中定居的潜力。

Tenacibaculum bacteria are found in coastal marine environments worldwide, including Canada. Several Tenacibaculum species have been shown to be opportunistic pathogens on cultured Atlantic salmon, however, there is a limited understanding of the marine ecology of this bacteria and of Tenacibaculum infections in Pacific salmon. This project aims to identify Tenacibaculum bacteria within and surrounding a Chinook salmon saltwater production site in British Columbia. The project will build on a strong partnership between University of Guelph (UGuelph), Vancouver Island University (VIU), Creative Salmon Company Ltd. and the BC Center for Aquatic Health Sciences (CAHS). The objectives of this project are to 1) collect and test water samples for Tenacibaculum species in and around Chinook salmon saltwater net-pen site; 2) collect and test Chinook salmon tissue for Tenacibaculum species during saltwater production; 3) collect and test biotic (invertebrates) and abiotic (net-pen infrastructure) organisms and surfaces at the saltwater production site for Tenacibaculum species and 4) utilize a high-throughput molecular assay to investigate Tenacibaculum species community distribution and relative abundance in collected samples. This project will provide an understanding of the presence of Tenacibaculum species in the wider marine environment and aide in our understanding for the potential of the bacteria to colonise pacific salmon.