Cost-effective Enzyme Booster Technology (EnBooT) for petroleum hydrocarbon biodegradation in cold regions

该项目的目的是开发一种新的绿色生物修复技术，酶促进技术（EnBooT），该技术可以以可承受的成本生产基于酶的配方特定地点鸡尾酒。现场定制的酶产品可以降低原生微生物在第一阶段的毒性。这项技术有可能克服其他修复方法的局限性，如原位热技术和/或原位化学氧化，但也将解决与寒冷气候地区生物修复相关的挑战，因为目前许多可用的原位修复解决方案在低温下无效。基于这项研究，加拿大安大略省和美国的生物修复公司和环境顾问将更好地了解EnBooT的市场适用性。像这样的技术可以为修复公司提供紧凑的系统，用于现场生产预配方的EnBooT，使他们能够针对不同的案例研究扩大酶的生产规模，并在不同的情况下展示新酶配方的功效。

The purpose of the project is to develop a new and green bioremediation technique, Enzyme Booster Technology (EnBooT), that can produce enzyme-based formulated site-specific cocktails at an affordable cost. Site-tailored enzyme products can reduce the toxicity of native microorganisms in the first stage. This technology has the potential to overcome the limitations of other remediation methods such as in-situ thermal technologies and/or in-situ chemical oxidation but will also address challenges associated with bioremediation in cold-climate regions, as many of the currently available in-situ remediation solutions are not effective at low temperatures. Based on this proposed research, bioremediation companies and environmental consultants in Ontario, Canada, and the U.S. will develop a better understanding of EnBooT's market applicability. Technology like this can provide remediation companies with compact systems for on-site production of pre-formulated EnBooT, enabling them to scale up enzyme production for different case studies and demonstrate the efficacy of new enzyme formulations in different scenarios.