LC-MS for radiochemistry and chemistry characterization

这项提议是为了在TRIUMF获得一台用于分析放射性和非放射性样品的液质联用仪(LC-MS)。目前，在TRIUMF还没有MS或核磁共振(核磁共振)仪器来直接进行化学表征。质谱学是化合物鉴定和反应监测的基本技术。对于放射性化合物，尽管现代化学长期以来需要多种直接表征，但其鉴定的传统做法仍然仅基于色谱(无线电-TLC和高效液相色谱)分析。在处理伽马排放低、难以检测到的放射性核素时，对层析信息的依赖变得更加不稳定。此外，如果衰变链中存在多个子离子，例如225Ac或227Th，则跟踪放射性产品的色谱图是复杂的。因此，对放射性化合物进行更彻底的表征有上升的趋势。为了跟上日益增长的科学标准，TRIUMF将从拥有LC-MS中获得巨大的好处，这将给我们提供一个急需的工具来识别或确认我们的化合物的组成。TRIUMF的研究人员将频繁使用LC-MS仪器。除了放射性化合物，它的使用还包括非放射性样品，使我们能够快速合成新的螯合剂和生物结合物。有了现场的LC-MS仪器，我们的学生参与合成项目的生产率将大大提高，帮助他们完成论文项目。我们的HQP还将受益于具有高度市场化的技能和动手操作LC-MS经验。几个由NSERC资助的项目将得到支持，包括四个以非常规放射性核素的螯合剂和生物偶合物开发为中心的DG项目。我们的大学合作伙伴没有安装LC-MS仪器来运行放射性化合物，也没有其他经济上可行的替代方案。因此，我们的LC-MS仪器将在TRIUMF内部以及来自21所成员大学和其他合作者的研究人员之间共享，为放射性化合物的光谱分析提供一个独特的平台，并帮助我们了解一些用传统方法难以表征的放射性核素的基础化学。所需的仪器要么是安捷伦的质量检测器，可以连接到我们现有的LC，要么是Waters的独立LC-MS系统。由于更高的灵敏度和更大的质量范围，安捷伦系统是首选。TRIUMF在安装需要核通风的仪器方面拥有丰富的经验。一名专门的仪器技术员将维护仪器并提供实践培训。简而言之，这些设备将提高放射性化合物表征的标准，产生更科学合理的结果，加深我们对放射性化合物的了解，支持NSERC资助的几个项目，并提供全面的HQP培训。

This proposal is to acquire a liquid chromatography - mass spectrometer (LC-MS) for analysis of radioactive and nonradioactive samples at TRIUMF. Currently, there is no MS or Nuclear Magnetic Resonance (NMR) instrument for direct chemical characterization at TRIUMF. MS is an essential technique for compound identification and reaction monitoring. For radioactive compounds, the conventional practice for their identification is still solely based on chromatographic (radio-TLC and HPLC) analysis, despite modern chemistry long requiring multiple direct characterizations. The reliance on chromatographic information becomes more precarious when working with radionuclides with low gamma emissions that are hard to detect. Furthermore, tracking the chromatograms of radioactive products is complicated if multiple daughter ions are present in the decay chain, such as with 225Ac or 227Th. Hence, there is a rising trend to obtain more thorough characterization of radioactive compounds. To keep pace with the increasing scientific standards, TRIUMF would hugely benefit from having an LC-MS, giving us a much-needed tool to identify or confirm the composition of our compounds. The LC-MS instrument would be frequently used by the researchers at TRIUMF. In addition to radioactive compounds, its use would involve nonradioactive samples, enabling us to fast-track the synthesis of new chelators and bioconjugates. Having an LC-MS instrument on-site, the productivity of our students involved in synthetic projects would be greatly enhanced, helping them with their thesis projects. Our HQPs would also benefit by having a highly marketable skill with hands-on LC-MS experience. Several NSERC funded programs will be supported, including four DG programs centered on chelator and bioconjugate development for unconventional radionuclides. There is no LC-MS instrument setup to run radioactive compounds by our university partners and there is no other financially feasible alternative. Thus, our LC-MS instrument will be shared among researchers within TRIUMF and from 21 member universities and other collaborators, providing a unique platform for spectrometric analysis of radioactive compounds and helping us understand the fundamental chemistry of some radionuclides that hard to characterize by conventional methods. The instrument required is either a mass detector from Agilent that can be connected to our existing LC, or a stand-alone LC-MS system from Waters. The Agilent system is preferred due to higher sensitivity and larger mass range. TRIUMF has extensive experience setting up instruments that need nuclear ventilation. A dedicated instrument technician will maintain the instrument and provide hands-on training. In short, the equipment will raise the standard for radioactive compound characterization, generate more scientifically sound results, deepen our knowledge of radioactive compounds, support several NSERC funded programs, and deliver well-rounded HQP training.