Connecting clubroot resistant canola to the clubroot pathogen virulence in East and West Canada

加拿大的根肿病管理在很大程度上依赖于抗根肿病品种的使用。不幸的是，多年来，抗性已被芸苔根肿菌（Plasmodiophora brassicae）的新致病型迅速克服，尽管也有证据表明有毒和无毒的芸苔根肿菌种群的复杂混合物共存，有毒种群最终占据田地并引起感染。通过精确了解田间致病型的多样性及其对商业卡诺拉品种的毒力，有可能定制抗性卡诺拉品种的选择，以在受侵染的田间使用尽可能长的时间。通过分析来自加拿大东部和西部的多种十字花科疫霉菌分离株的基因组，我们将能够识别特定的靶点，可用于识别它们是有毒的还是无毒的。我们的目标是开发一种分子检测方法，可以快速准确地鉴定无毒致病型，并为每个种植者鉴定合适的抗性种质，避免与疾病相关的经济损失，以及基于化学品的管理对环境的影响。

Clubroot management in Canada relies heavily on the use of clubroot resistant varieties. Unfortunately, over the years, the resistance has been rapidly overcome by new pathotypes of Plasmodiophora brassicae although there is also evidence that complex mixes of virulent and avirulent P. brassicae populations co-exist, with virulent populations eventually taking over a field and causing infection. With precise knowledge of the diversity of pathotypes in the field and their virulence towards commercial canola varieties it would be possible to tailor the selection of resistant canola varieties to use in an infested field for the longest possible time. Through the analysis of the genome of a wide array of P. brassicae isolates from East and West Canada we will be able to identify specific targets that can be used to identify if they are virulent or avirulent. Our goal is to develop a molecular assay that allows fast and precise identification of avirulent pathotypes and identify the suitable resistance germplasm for each grower and avoid economic loses linked to the disease, and the environmental impact of the chemical-based management.