The clubroot pathogen: a master manipulator of plant immunity?

根肿病病原体：植物免疫的主宰者？

• 批准号: 576141-2022
• 负责人: PerezLopez, EdelE
• 金额: $ 1.82万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=763517
• 关键词: clubroot; pathogen; master; manipulator; plant

Since 2020, thanks to the support of the NSERC through the Discovery Program, as well as the support of canola (Brassica napus) grower's associations, our Lab (EdelLab) at Université Laval (UL) is trying to understand the molecular basis of the clubroot resistance and how the clubroot pathogen can infect susceptible hosts. Clubroot is a devastating disease infecting roots of plants in the Brassicaceae family and responsible for 10-15% yield reduction on a global scale, and more than $ 500 million in loses to the Canadian canola industry in the past 10 years. This disease is caused by Plasmodiophora brassicae, an obligate intracellular biotrophic plant pathogen that belongs to the class Phytomyxea within the eukaryote supergroup Rhizaria, one of the least studied groups of eukaryotes. Clubroot management in Canada relies heavily on the use of clubroot resistant varieties. Unfortunately, over the years, the resistance has been rapidly overcome by new pathotypes of P. brassicae, situation that is aggravated by the fact that very little is known about the identity of the clubroot resistant genes or the mechanisms mediating the resistance, and how the clubroot pathogen can suppress plant immunity in the susceptible host. The main objective of this international collaboration is to advance our efforts in identifying putative clubroot resistance genes in canola and Arabidopsis for further validation and characterization. This will be addressed through the use of resistance gene enrichment sequencing (RenSeq), genome-wide association studies (GWAS), and CRISPR-Cas9-based reverse genetics, all technologies well-known and established in DingLab lead by Dr. Pingtao Ding at the Institute of Biology Lieden, at Lieden University, Netherland. This collaboration will contribute to the training of two PhD students and will ensure the Canadian cruciferous industry more stable and robust clubroot resistance.

自2020年以来，得益于NSERC通过发现计划的支持，以及油菜（油菜）种植者协会的支持，我们在拉瓦尔大学（UL）的实验室（EdelLab）正试图了解根肿病抗性的分子基础以及根肿病病原体如何感染易感宿主。根肿病（Clubroot）是一种危害油菜科植物根部的毁灭性病害，在全球范围内造成10-15%的减产，在过去10年中给加拿大油菜产业造成了超过5亿美元的损失。这种疾病由芸苔根肿菌（Plasmodiophora brassicae）引起，芸苔根肿菌是一种专性细胞内生物营养型植物病原体，属于真核生物超组Rhizaria（真核生物研究最少的组之一）内的Phytomyxea纲。加拿大的根肿病管理在很大程度上依赖于抗根肿病品种的使用。不幸的是，多年来，抗性已被新的芸苔根肿病致病型迅速克服，由于对根肿病抗性基因的身份或介导抗性的机制以及根肿病病原体如何抑制易感宿主中的植物免疫力知之甚少，情况变得更加严重。这项国际合作的主要目标是推进我们在鉴定油菜和拟南芥中推定的根肿病抗性基因方面的努力，以进一步验证和表征。这将通过使用抗性基因富集测序（RenSeq），全基因组关联研究（GWAS）和基于CRISPR-Cas9的反向遗传学来解决，所有这些技术都是众所周知的，并在DingLab中建立，由Dingtao Ding博士领导。这项合作将有助于培养两名博士生，并将确保加拿大十字花科产业更加稳定和强大的根肿病抗性。