FGF20对毛囊干细胞调控及促进毛囊再生作用机制研究

雄激素性脱发、创伤后瘢痕性脱发等脱发问题亟待解决，但组织工程皮肤中毛囊再生问题尚未解决，如何有效调节毛囊干细胞增殖分化并促进毛囊再生值得研究。近期有研究发现FGF20在毛发发育中起决定性作用。我们前期也发现FGF20可促进培养毛囊生长，明显促进创伤诱导毛囊再生并上调Wnt信号通路表达水平，但FGF20调节毛囊生长/再生的具体作用机制尚不清楚。本项目拟采用毛囊器官离体培养模型、创伤诱导毛囊再生模型、敲除Fgfr转基因动物的创伤诱导毛囊再生模型、Patch Assay在体毛囊再生模型等，从不同角度不同水平探究FGF20对毛囊干细胞/毛囊前体细胞增殖分化调节作用、促进毛囊生长作用和诱导毛囊再生作用，尤其探究FGF20作用受体及FGF20/Fgfr/Wnt信号通路在其作用机制。本项目有望获得有效促进毛囊生长/再生药物，为解决毛囊再生这一组织工程问题奠定基础，为脱发临床治疗提供新思路。

Hair loss, caused by androgenetic alopecia or wounds, is a common clinical condition and severely affects patients' quality of life. Hair follicle regeneration is a challenging task in regenerative medicine. Despite of our current knowledge in hair follicle stem cells, more studies are needed to understand the molecular regulations of their regenerative capacity. Many recent trials demonstrated the decisive effects of FGF20 in the development of hair follicles. Our preliminary studies also confirmed that FGF20 enhanced the growth of cultured hair follicles, promoted wound induced hair morphogenesis, and up-regulated gene expression in Wnt singaling pathway. These data suggested FGF20 plays an important role in hair follicle morphogenesis and growth, however, the exact mechanisms by which FGF20 regulates hair regeneration in adults remain unknown. We plan to use ex vivo hair follicles culture model, wound induced hair morphogenesis model, Fgfr knock-out mice wound model, and Patch Assay hair follicle organogenesis model to study the regulatory effect of FGF20 on hair follicle stem/progenitor cells and regeneration. Especially, we focus on the mechanisms of FGF20/Fgfr/Wnt signalling pathway. This project will help to elucidate the role of FGF20 in hair follicle regeneration, and lead to potential clinical applications of FGF20 for the treatment of hair loss.