小麦TaNRC1基因调控细胞凋亡结构域鉴定及其抗病机制解析

小麦条锈病和白粉病是小麦生产的两个重要病害，严重影响着小麦生产。在抗病基因遗传基础日渐狭窄和病菌毒性变异的背景下，解析小麦与病菌互作机理，有针对性持久控制病害显得尤为迫切。在前期利用YrSM139-1B抗病基因精细作图的基础上获得了TaNRC1基因。该基因在小麦-条锈菌互作中上调表达，沉默后可增强小麦感病性；在烟草中过表达TaNRC1基因变体具有自发性激发HR功能，即其具有通过改变结构增强小麦抗条锈病接触抗性能力。本研究欲利用AS-PCR技术分析该基因在抗感近等基因系中剪切体表达特征，利用人工突变创制结构域缺失变体系、结合瞬时表达明确各变体的结构域功能；借助CoIP-MS技术和酵母双杂系统筛选其互作蛋白并构建调控网络；进而利用小麦转基因技术对其抗性功能进行功能验证，以期深入解析该基因各功能结构域在细胞凋亡功能中的角色和具体功能，揭示TaNRC1基因致使细胞凋亡的作用机制。

Stripe rust and powdery mildew severely affect the security of wheat production in China. The rapid virulence variation of pathogen results in ‘resistance loss’ of wheat cultivars, and aggravate the disease risk of wheat production. So it is particularly urgent to reveal the mechanism of wheat-fungi interactions for durable disease control pertinently. In previous studies, we identified a NB-ARC genes, named as TaNRC1, by Race on the basis of STS marker of YrSM139-1B stripe rust resistance loci. TaNRC1 were significantly induced during the incompatible wheat-Pst interactions. Silencing of them enhanced the susceptibility of wheat to Pst, while the splice variants could regulate the hypersensitive response (HR) in Nicotiana benthamiana. These indicating that TaNRC1 enhance the plant resistance to pathogens via changing the protein structure. Thus, in this project, we plan to characterize the expression of splice variants by AS-PCR comparing resistance with susceptible NILs, and further study the detailed function of it’s protein domains using transient expression in tobacco. In addition, we aim to select the NRC1-assossiated proteins by CoIP-MS and Y2H method, and finally analyze the regulation roles of TaNRC1 in cell death in the resistance interaction of wheat and fungi. Our results will reveal the modulation mechanism of TaNRC1 cell death activation, and lay the foundation for clarifying the mechanism of wheat and fungi interactions, which will furthermore provide gene resource, theoretical basis to control disease using new strategies.