葡萄microRNA398及其靶基因响应波尔多液中Cu2+的机理

葡萄(Vitis vinifera)是世界性的重要果树，也是容易遭受病害的果树树种之一。波尔多液是葡萄病害防治中使用最早、最普遍，也是应用效果很好的含铜杀菌剂。人们满足于波尔多液杀菌的功效时，却忽略了波尔多液对葡萄树体本身的影响以及葡萄对喷施波尔多液后出现的过多外源Cu2+的响应。鉴于microRNA398（miR398）及其靶基因在调节植物铜代谢平衡、应答铜胁迫过程中扮演的重要角色。本研究拟通过Western杂交、定量RT-PCR、PPM-RACE和RLM-RACE技术、miR-RACE技术等，明确Vv-miR398对靶基因的调控方式以及Vv-miR398与靶基因应答葡萄外源铜胁迫的生物机制。这对了解Vv-miR398及其靶基因在葡萄铜胁迫应答网络中的作用与葡萄抗铜胁迫能力至关重要，对于更好地利用波尔多液服务于葡萄乃至农林生产等具有重要理论指导意义。

Grape (Vitis vinifera) is an important fruit tree in the world. It is also one of the fruit tree species vulnerable to various diseases. Bordeaux mixture, one of the most common copper fungicides, was first and commonly applied in the grape disease prevention with good effect. It is noticed that people overlooked the influence of Bordeaux mixture to grape trees and the response of grape trees to extra Cu2+ in the spray. MicroRNA398 (miR398) and its target genes play a vital role in the process of regulating copper metabolism balance and response to the copper stress. Based on the mentioned above, this research is designed to understand Vv-miR398's regulation on its target genes and the role both of Vv-miR398 and its target genes in responding exogenous copper stress, for which Western blotting, qRT-PCR, PPM-RACE and RLM-RACE, and miR-RACE technologies will be applied. This research is important for our understanding the role of Vv-miR398 and its target genes in copper stress response network of grape and resistance to copper stress. Its accomplishment can definitely provide significant theory for the better use of Bordeaux mixture in grape and even in agricultural and forestry production.