基于“纳米晶-重组抗体”微阵列免疫芯片的中药材上农药多残留检测新方法研究

中药材种植过程中三嗪类除草剂的长期大量使用易导致多农药残留，高效、低成本的现场检测已成为中药安全研究领域和监管部门的迫切需求。借助重组抗体能特异识别待测目标，基于纳米晶标记的微阵列免疫芯片技术仅需少量样品即可实现多目标成分的实时、灵敏检测。本项目拟在前期合成可调控纳米晶的基础上，重点针对毒性大、半衰期长的三嗪类除草剂，采用计算机辅助3D分子模拟技术设计并合成群选性半抗原，通过噬菌体抗体库技术筛选适合中药材检测的重组抗体，配以微阵列免疫芯片技术，建立基于“纳米晶-重组抗体”的中药材三嗪类除草剂现场高通量检测的便携式微阵列芯片扫描仪分析新方法。同时佐以UPLC-MS/MS 的实验室检测结果，确证新方法在中药材上的准确性和普适性，为中药材农药多残留的现场检测提供数据和技术支持。该项目的实施对消除中药材农药残留的潜在安全隐患、突破国外贸易壁垒，增加我国中药材出口创汇，具有重要的理论价值和现实意义。

Long-term and numerous use of triazine herbicides on traditional Chinese medicines (TCMs) in the process of planting can easily cause multi-pesticide residues, affecting the quality and safety of them. Therefore, on-site detection of triazine herbicides with little time and low cost has been the urgent requirement of the TCM research fields and the supervision departments. Recombinant antibody has the ability of identifying the targets with good specificity and high affinity. Microarray immunochip technique labeled by nanocrystal has the advantage of simultaneously and sensitively detecting the targets in a small amount of sample. Based on the preparation of controlled nanocrystal, and triazine herbicides with high toxicity and long half-time in TCMs as the objects, we aimed to design and synthesize the selected hapten by using the computer-aided 3D molecular molecular modeling technology, and to screen the recombinant antibody for specially detecting triazine herbicides in TCMs through phage antibody library technology, then, together with microarray immunochip technique based on "nanocrystal-recombinant antibody" to establish a novel method suitable for the small-sized, portable microarray chip scanner for on-site high-throughput detection of triazine herbicides in TCMs. Through comparing the results of on-site analysis using the novel established method and indoor detection using UPLC-MS/MS system by statistical analysis, the accuracy and universality of the novel method for more TCMs would be confirmed. The study would provide data and technique supports for on-site rapid detection of multi-pesticide residues in TCMs. In summary, this project express important theoretical and practical significance for eliminating potential security risks of pesticide residues in TCMs, and for breaking the foreign trade barriers, further for increasing exports of more TCMs.