生物相容性币金属纳米团簇的制备、结构表征及其在检测“活性硫物种”中的应用

针对目前广泛使用的有机荧光试剂合成复杂、细胞毒性大、生物相容性低，同时在荧光分析法中还存在背景荧光的干扰等难点、热点问题，本项目参照配体性质的变化引起币金属纳米团簇性质的改变这一规律，拟设计、合成以胞嘧啶核苷为配体保护的具有生物相容性和手性的币金属纳米团簇，发展圆二色谱检测“活性硫物种”的新手段。采用X-ray单晶衍射、核磁共振、质谱、元素分析及红外光谱等方法对目标团簇进行结构表征后，研究其结构与性能的相关关系，再结合其在检测“活性硫物种”过程中溶液圆二色信号的变化，提出与实验结果一致的作用机理。以上成果将进一步揭示币金属纳米团簇的界表面结构，摆脱荧光分析法必须基于发光化合物的限制，建立起基于银纳米团簇的圆二色光谱分析法，为解决生理条件下检测“活性硫物种”的过程中背景干扰问题提供新的思路和方案。

Organic fluorescence reagents are widely used in every aspect, but there are problems with the difficulties in preparation, high cytotoxicity, low biocompatibility and the interference of background fluorescence in fluorimetry. According to the rule that the character change of the periphery ligand may lead to the modification of the whole coinage metal nanoclusters, this project try to design and synthesis the cytidine-protected coinage metal nanocluster to transform their biocompatibility and chirality. The convenience method are explored for the preparation of stable coinage metal nanoclusters and a new method for the physiological detection of reactive sulfur species (RSS) by circular dichroism spectra (CD) is developed. The target clusters will be characterized by single-crystal X-ray diffraction, NMR, MS, EA and IR. The relationship between their molecular structures and properties will be studied. The special CD properties of coinage metal nanoclusters could be used for the detection of RSS with CD signal alterations. The rules and mechanism of the interaction between them proposed in line with the experimental results will be felt out. The results will further reveal the surface and the interface of coinage metal nanoclusters and provide a new CD analysis method based on the cytidine-protected silver nanocluster, which can free fluorometry from the constraint that the used compound must be luminous. And it also helps to provide a new solution for the interference problem of background in the physiological detection of RSS.