矮牵牛转录因子PhDof4参与花药发育调控的分子机理研究

花药是矮牵牛重要的生殖器官，对其育性有决定性作用，然而矮牵牛花药发育分子调控的机制至今仍鲜有报道。前人对140个水稻花药特异性表达基因启动子中的顺式调控元件进行了分析，发现DOFCOREZM为其中普遍存在的调控元件，暗示着Dof转录因子家族成员在花药发育过程中可能具有重要的作用。在前期研究中，我们对矮牵牛Dof转录因子家族中23个成员的组织特异性表达情况进行了鉴定，发现了一个在花药中优势表达的候选Dof基因PhDof4，而且其表达水平在花药绒毡层降解阶段具有显著提高的趋势。本研究拟先通过转化矮牵牛来确认PhDof4在花药发育过程中的功能，再通过转录组测序、酵母单杂交等技术，对PhDof4调控的下游基因以及其上游调控因子进行筛选，初步解析PhDof4在矮牵牛花药发育过程中的表达调控机理，并为矮牵牛雄性不育系的创造挖掘新的基因资源。

As the most important male reproductive organ, anthers can directly control the fertility of petunia. However, there is only a little reports about the molecular regulation mechanism of petunia anther development. In the previous study, according to the biological information analysis of 140 rice anther-specific genes’ promoters, the DOFCOREZM was identified as one of the common cis-elements, implying that the Dof transcription factor family members could play important regulation roles in the anthers. In our previous research, the tissue-specific expression patterns of 23 Dof transcription factor family genes were analysed in petunia. We found PhDof4 was an anther-preferential expressed gene and significant highly expressed in the anther tapetum degradation stage. Therefore, we plan to clarify the function of PhDof4 in anthers by transforming petunia 'W115', and then explore the up/down-stream genes of PhDof4 by transcriptome sequencing and yeast one-hybrid technologies, so that we can preliminary explain the expression regulation mechanism of PhDof4 during anther development, and provide new gene resources for creating petunia male-sterile lines.