基于生化遗传过程的大麦籽粒蛋白质形成机理模型构建

蛋白质是大麦重要品质指标，大麦蛋白质形成模型是构建大麦生产管理决策支持系统、实现大麦生产精确化和数字化迫切需要解决的关键技术之一。基于生化遗传过程构建的大麦籽粒蛋白质形成的机理模型，能更深刻地反应蛋白质形成的实质，机理性更强，目前关于此方面的研究尚属空白。本研究以源库理论为框架，以籽粒蛋白质形成的生化遗传机制为基础，以蛋白质的合成底物游离氨基酸含量为纽带，以调控蛋白质合成关键酶-谷氨酰胺合成酶基因(HvGS1)相对表达量变化规律为主线，描述籽粒蛋白质的合成过程；以不同氮肥处理水平与HvGS1相对表达量的相关性表达施氮肥水平对籽粒蛋白质形成的影响。在此基础上构建基于生化遗传过程的大麦籽粒蛋白质形成的机理模型，并与我们先期构建的基于源库理论的大麦籽粒蛋白质预测模型进行比较，为大麦生产中籽粒蛋白质含量的动态预测提供量化工具，也为从分子水平构建作物模型提供一条新的思路。

Protein is an important quality indicator of barley grain.The construction of barley quality model is one of the key technologies in the field of barley simulation and decision support system of barley production. Its mechanical model can reflect the nature of protein formation in barley grain based on biochemical genetic processes.However, these kinds of researches are still blank in this area.This investigation establishes a source-sink relationship as its framework, sets up the biochemical genetic processes of barley grain protein formation as its foundation, investigates the amount of free amino acid in protein synthesis, depicts the relative expression level of variation pattern of the key enzyme gene, glutamine synthetase (GS1) gene HvGS1, in the regulation of protein synthesis as its core. This study will also exam the impact of the formation of nitrogen fertilizer level on grain protein according to different nitrogen treatments based on correlation of HvGS relative expression and the amount of grain protein. Compared with our former experiences of our pre-constructed barley grain protein model, the current model will lay foundation for building the barley production system, and will provide a new way of thinking in constructing crop growth and quality formation model on molecular level.