一种油桐LRR类抗枯萎病基因vfLRR的分离与功能鉴定

油桐枯萎病是油桐病虫害中最严重的一种毁灭性病害，初期较难鉴别，防治效果不理想，发病机制尚不明确。挖掘油桐抗枯萎病基因，对阐明油桐抗枯萎病分子机制和基因工程抗性育种有重要科学指导作用。本项目在近两年赴病区调研、分离获得油桐枯萎病病原菌Fusarium oxysporum专化型，及初步筛选获得油桐LRR类抗病基因vfLRR基础上，拟5'RACE获得基因全长；通过油桐苗病原菌接种处理、实时定量PCR技术和荧光融合蛋白技术分析油桐抗枯萎病反应中vfLRR的响应规律；通过vfLRR补偿拟南芥突变体实验，揭示vfLRR表达对植株抗枯萎病反应能力的影响；通过拟南芥双突植株的vfLRR转化实验，确定vfLRR介导的抗枯萎病反应是否依赖于植物抗性NPR1信号通路。通过上述研究，阐明油桐vfLRR基因在抗油桐枯萎病反应中的功能，丰富油桐抗病遗传育种工程中重要遗传资源，为深入阐明油桐抗枯萎病反应机理奠定基础。

Tung fusarium wilt is the worst disease, and had been serious in the last century and recently. Our standing of tung tree Fusarium wilt resistance mechnism is sill in its infancy. Resistance gene acquisition is important for clarification of tung tree Fusarium wilt resistance mechnism.On the basis of investigation and isolation of tung Fusarium oxysporum,and screening for obtain vfLRR gene with leucine-rich repeats from tung,the project aimed to study F.oxysporum influence on vfLRR expression by employing inoculation, qRT-PCR and DsRed protein fuse techniques; clarify the effect of vfLRR on Fusarium wilt resistance by gene compensation experiment; and indentify whether the effect of vfLRR on F. oxysporum dependent on NPR signal pathway through transformation of Arabidopsis thaliana double mutant(atlrr, atnpr1). The study indicated to identify the vfLRR function in tung fusarium wilt resistance and enrich the gene resources for tung disease resistance breeding, which held promise for clarifying tung fusarium wilt resistance mechnism.