无芒隐子草闭花授粉机制研究

我国90% 的草地已不同程度退化，充分利用能适应当地环境的的乡土草资源（如旱生性无芒隐子草），是改良退化草地、改善生态环境的有效之途。我们的前期研究发现，无芒隐子草同时存在闭花授粉（CL）和开花授粉（CH），CL小穗的种子产量约占总产量的82%，但造成这种差异的机制尚未阐明。本项目拟进一步研究CL和CH小穗、花器官的形态学、细胞学基础；首次以CL家族植物无芒隐子草整合基因组DNA测序、RNA转录组和Small RNA测序研究其CL的分子动态，验证10个以上CL相关cDNA和Small RNA的生物学功能以及CL与抗旱基因的关联性；从而揭示无芒隐子草CL和CH繁殖分配，CL的细胞和分子机理。为无芒隐子草新品种的种子生产和推广利用提供技术支撑，也为被子植物CL机制奠定理论基础。

In China, the 90% of total grasslands are degraded under a very degree. The native grasses, such as Cleistogenes songorica, which adapt the local environment very well, are important foundation for reclamation of degraded grasslands and improvement of environment. Our previous research results showed that the cleistogamous (CL) and chasmogamous (CH) are existed contemporarily in C. songorica. The seed yield in CL are contribute of 82% of total seed yield in C. songorica. Up to now, the mechanism of CL is still unclear. Further research will concentrate on morphology and cytology of the spikelet and floral organs in both CL and CH. For the first time, transcription Unigenes will be released in C. songorica based on intergrating genomic DNA sequencing, RNA transcriptome and Small RNA sequecing of floret. Meanwhile, more than 10 novel cDNA genes and Small RNAs related with CL should be functionly identified and reveal the correlationship between CL and drought stress among these Unigenes. The results will illuminate the reproductive allocation between CL and CH in C. songorica, also the cytology and molecular mechanism of CL. These work will provide the technical support for the seed production and extension of C. songorica cultivar, will also increase the understanding of CL in angiosperm.