Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica
Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica
复制标题
bZIP转录因子的综合分析揭示了它们在隐子草二态性小花分化和胁迫反应中的作用
DOI：
10.1186/s12864-019-6092-4
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发表时间：
2019-10
期刊：
影响因子：
4.4
通讯作者：
Jiyu Zhang
中科院分区：
文献类型：
--
作者：
Qi Yan;Fan Wu;Tiantian Ma;Xifang Zong;Qian Ma;Jie Li;Yufeng Zhao;Yanrong Wang;Jiyu Zhang
背景
转录因子是转录网络的重要调节因子。碱性亮氨酸拉链 (bZIP) 转录因子已被证明参与植物的多种生物过程。然而，对于荒漠草原中重要的旱生异源四倍体草类隐子草bZIP家族，目前还没有任何信息。
结果
在这项研究中，在异源四倍体 C. songorica 基因组中鉴定出了 86 个 CsbZIP。为了进行位置分析，CsbZIP 均匀分布在 C. songorica 的两个亚基因组中。三个物种的系统发育树分析表明，CsbZIPs 在进化上与 OsbZIPs 的关系比 AtbZIPs 更密切。同线性和系统发育分析证实，CsbZIP 主要通过全基因组复制事件进行扩展。此外，通过计算直系同源基因对的Ks值和Ka/Ks比值，确定水稻和松果在其漫长的进化历史中可能经历了纯化选择。通过分析CsbZIPs在不同组织和非生物胁迫下的表达模式，发现21个CsbZIP基因在雌雄花（CH）和闭锁花（CL）之间差异表达，其中包括两个FLOWERING LOCUS D（FD）基因。在芽和根中，分别有 79.1% 和 87.2% 的 CsbZIP 基因在至少一种胁迫处理（例如热、冷、干旱和盐）下表现出转录本变化。引人注目的是，17 个常见的 CsbZIP 基因在胁迫反应和 CL 开花期间表现出差异表达。共表达网络、GO注释和实时定量逆转录PCR（qRT-PCR）分析揭示了CL开花相关基因和非生物胁迫相关基因之间的密切关系。
结论
在异源四倍体 C. Songorica 中对 BZIP TF 进行了全面分析和鉴定。我们的结果提供了对桑戈卡 bZIP 家族进化历史的深入了解，并为非生物胁迫响应和 CL 相关候选 CsbZIP 基因提供了在植物遗传改良中的潜在应用。
Background
Transcription factors act as important regulators of transcription networks. Basic leucine zipper (bZIP) transcription factors have been shown to be involved in multiple biological processes in plants. However, no information is available for the bZIP family in Cleistogenes songorica, which is an important xerophytic and allotetraploid grass in desert grasslands.
Results
In this study, 86 CsbZIPs were identified in the allotetraploid C. songorica genome. For location analysis, CsbZIPs were distributed evenly across two subgenomes of C. songorica. Phylogenetic tree analysis among three species indicated that CsbZIPs were evolutionarily more closely related to OsbZIPs than AtbZIPs. Syntenic and phylogenetic analyses confirmed that the CsbZIPs were mainly expanded by whole-genome duplication events. Furthermore, it was determined that rice and C. songorica might have undergone purified selection during their long evolutionary history by calculating the Ks values and Ka/Ks ratios of orthologous gene pairs. By analysing the expression patterns of CsbZIPs in different tissues and under abiotic stresses, 21 CsbZIP genes were differentially expressed between chasmogamous (CH) and cleistogamous (CL) flowers, including two FLOWERING LOCUS D (FD) genes. In shoots and roots, 79.1 and 87.2% of the CsbZIP genes, respectively, displayed transcript changes under at least one stress treatment, such as heat, cold, drought and salt. Strikingly, 17 common CsbZIP genes showed differential expression under stress response and during CL flowering. Co-expression network, GO annotation and real-time quantitative reverse transcription PCR (qRT-PCR) analyses revealed a close relationship between CL flowering-associated genes and abiotic stress-related genes.
Conclusions
BZIP TFs were comprehensively analysed and identified in allotetraploid C. songorica. Our results provide insights into the evolutionary history of the bZIP family in C. songorica and provide abiotic stress-responsive and CL-associated candidate CsbZIP genes for potential applications in the genetic improvement of plants.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
5.6
作者：
Li X;Fan S;Hu W;Liu G;Wei Y;He C;Shi H
通讯作者：
Shi H
影响因子：
14.9
作者：
Zhao K;Liu M;Burgess RR
通讯作者：
Burgess RR
影响因子：
2.9
作者：
Wang XL;Chen X;Yang TB;Cheng Q;Cheng ZM
通讯作者：
Cheng ZM
DOI：
--
发表时间：
2001
期刊：
Journal of Arid Land Resources and Environment
影响因子：
--
作者：
Yang Jing
通讯作者：
Yang Jing