基于“谱-效”关系和SCF/c-kit信号通路研究黄精赞育胶囊治疗少弱精子症的物质基础及作用机制

少、弱精子症是男性不育领域研究的热点和难题。课题组前期以黄精赞育胶囊干预少、弱精子症取得了良好的临床疗效。本研究根据中药复方“谱-效”关系理念，结合SCF/c-kit信号通路调控生精细胞凋亡机制的最新认识，提出假说：黄精赞育胶囊通过有效成分谱调控SCF/c-kit信号通路的整体功能治疗少、弱精子症。拟采用信息融合技术建立黄精赞育胶囊体外指纹图谱与体内血清药物化学相结合的HPLC-DAD和HPLC-ELSD指纹图谱；并检测黄精赞育胶囊对少、弱精子症大鼠SCF/c-kit信号通路的整体变化。整合以上研究结果，建立黄精赞育胶囊调节SCF/c-kit信号通路的“谱-效”关系，旨在科学阐释黄精赞育胶囊治疗少、弱精子症的有效靶点和作用机制，为临床合理用药提供依据，对于指导中医药治疗男性不育具有重要意义。

Oligoasthenospermia has long been the most discussed subject and remains as a problem in andrology. Huang Jing Zan Yu Capsule Capsule, a kind of spermatogenic prescription developed by our team for the treatment of sterility , has demonstrated positive therapeutic effect clinically. Based on the rationale of “ Spectrum-effect relationship” of readily-made Chinese medicine and new findings on the role of SCF/c-kit signal pathway in regulating the generation and apoptosis of sperm cells, it is hypothesized that active components in Huang Jing Zan Yu Capsule is likely to be a cure for Oligoasthenospermia throughout its regulation on the SCF/c-kit signal pathway. Aided with information technology,HPLC-DAD and HPLC-ELSD fingerprinting are established to investigate the regulatory effect of Huang Jing Zan Yu Capsule to mice with oligoasthenospermia. The “ Spectrum-effect relationship” of Huang Jing Zan Yu Capsule is also developed based on our previous research findings, with an attempt to pinpoint the valid target for treatment for oligoasthenospermia and its action mechanism,providing reference for clinical rational use of drug and guiding the treatment of infertility with the use of direct traditional Chinese medicine.