aurJ3M基因对新型食品防腐剂金褐霉素代谢网络的调控机制研究

金褐霉素（Aureofuscin）是一种四烯大环内酯类抗真菌物质，可有效防治水果采后病害，是一种具有很好的应用前景的高效、安全的新型生物防腐剂。但由于金褐霉素生物合成途径及其代谢调控机制尚不明确，难以采取相应有效措施提高其产率。我们在前期研究中已克隆到基因aurj3M，并发现其具有促进金褐霉素生物合成的作用，但其功能及调控机制需要进一步阐明。本项目拟采用基因敲除以及过表达技术阐明aurJ3M基因的功能；利用RT-PCR技术以及GST-PAS融合蛋白表达技术研究aurJ3M基因对金褐霉素生物合成中PKS途径的特异性调控作用；从发酵代谢节点进一步研究前体诱导物对aurJ3M基因调控金褐霉素合成的影响。本项目可望从基因组和蛋白质层次深入阐明aurJ3M基因对金褐霉素代谢网络的调控机制，以期为通过基因改造和结构修饰提高金褐霉素发酵产量提供理论依据。

Aureofuscin is a tetraene macrolides antifungal antibiotic and can inhibit postharvest disease of fruit, showing high performance, safety and stable biological antiseptic with promising application. However, Aureofuscin has not been achieved industrialization requirement yet because its biosynthesis and regulation mechanism is obscure. Base on our previous research work that the gene aurJ3M which can enhance Aureofuscin biosynthesis was cloned from Streptomyces aureofuscus. We attempt to clarify the regulation mechanism of gene aurJ3M on the metabolic network of Aureofuscin from the genome and protein level from three aspects: 1) analyze the function of gene aurJ3M by the technology of gene knockout and over-expression, 2) clarify the specific regulation mechanism of gene aurJ3M in the PKS pathway of Aureofuscin biosynthesis by the technology of RT-PCR and GST-PAS Expression of fusion protein, and 3)acquire the influence of precursor inducer on gene aurJ3M in Aureofuscin biosynthesis. This item may provide new knowledge about the production of Aureofuscin by reforming gene or structural modification.