铜基金属有机多面体组装体模拟酶在生化分析中的应用研究

因其具有化学性质稳定、结构简单、廉价易得等优点，模拟酶的研究越来越受到人们重视，成为目前化学科学一个重要研究领域。然而目前所开发的模拟酶普遍存在催化选择性不高的问题，限制了其在分析化学中的进一步应用。本项目拟以铜基金属有机多面体为基本单元，通过对其有机配体进行修饰改造，引入对生物分子具有特异性识别能力的功能基团，通过可控组装，建立同时具有天然酶活性部位和底物结合部位的自组装模拟酶；以铜基金属有机多面体模拟酶的生物催化性质及其表面特异性识别位点为基础，将此类组装体应用于化学发光、比色法、电分析化学检测等领域，建立以生物分子为检测对象的一些简便、快速、高灵敏度的分析检测方法。项目的开展有望深入探讨铜基金属有机多面体对生物分子催化的机理，探索模拟酶物质组成、结构与对其性能影响规律，为设计高选择性模拟酶提供理论基础，建立一些生物分子的高选择性检测方法，拓展金属有机多面体的应用领域。

Mimic enzymes have attracted more and more attentions and became a new research topic by the virtue of their good chemical stability, simple structure and inexpensive properties. The further application of mimic enzymes in analytical chemistry was hampered by their low catalytic selectivity. Thus, it is a critical issue to build mimic enzymes with high catalytic selectivity. In this project, we will prepare mimic enzymes by assembling of metal-organic polyhedra （MOP）with catalytic activity and binding site through introducing some groups with high recognition capability towards bio-molecule. Some facile, highly selective and sensitive chemiluminescent, colorimetry and electrochemical analysis methods targeted for biomolecules can be built based on the mimic enzyme activity of Cu-MOP. This project can develop some new mimic enzymes with selectively catalytic activity to deeply investigate the related catalytic mechanism of Cu-MOP and expand related applications in analytical chemistry.