microRNA-17-92cluster通过mTORC1/decorin通路调控毛囊干细胞微环境及生长期启动的作用研究

毛囊干细胞（HFSC）的活化在毛囊由休止期向生长期转化中发挥关键作用，microRNA (miRNAs)对毛囊周期的调节已日益引人关注。课题组在前期研究发现HFSC部位敲除miR-17-92cluster能促进小鼠休止期毛囊进入生长期的基础上，结合靶基因预测推测miR-17-92cluster通过mTORC1/decorin通路参与HFSC及毛囊周期调控。本项目拟通过对前期构建的HFSC部位miR-17-92cluster敲除小鼠进行组织学分析、药物干预实验及回补实验等在体研究，结合通路抑制剂处理及功能研究等体外实验，明确miR-17-92cluster通过mTORC1/decorin通路调控HFSC niche及生长期启动的作用及机制。本研究将为阐明miRNAs诱导毛囊再生的作用及机制提供新线索，为合理利用HFSC治疗脱发等疾病提供新靶点。

Telogen-anagen transition begins when the hair follicle stem cells (HFSCs) are activated. Recently, more and more attentions have been drew with the character of microRNAs (miRNAs) in controlling hair cycle. Based on our previous research that miR-17-92 cluster knockout in HFSC promoted telogen-anagen transition and the target gene prediction results, we provide the hypothesis that miR-17-92 cluster regulate HFSC and hair follicle cycle via mTORC1/decorin pathway. By utilizing the conditional miR-17-92 cluster knockout mouse, we will perform in vivo histological assessment, pharmacological intervention, rescue experiment, in vitro signaling inhibitor treatment and function study to reveal the role of miR-17-92 cluster in regulating HFSC niche and anagen initiation via mTORC1/decorin signaling. Our study will provide new clues to elucidate the role and mechanism of microRNAs in regulating follicular reconstruction, and provide new therapy targets for use of HFSC in the treatment of alopecia.