水稻条纹花叶病毒激活介体昆虫多胺合成通路促进病毒侵染的机制

水稻条纹花叶病毒（Rice stripe mosaic virus, RSMV）是最新发现并由介体昆虫电光叶蝉传播的胞质型弹状病毒。RSMV侵染可显著增加昆虫体内的多胺含量，并诱导昆虫多胺合成通路鸟氨酸脱羧酶抗酶（OAZ）上调表达，而且OAZ与RSMV的M蛋白发生特异性互作，推测RSMV可能通过调控多胺合成通路促进其在昆虫体内的增殖。本项目拟以介体电光叶蝉传播的RSMV为主要研究对象，利用电光叶蝉培养细胞体系，结合细胞生物学、电镜切片、免疫荧光标记和RNAi等技术体系，研究RSMV在介体中通过激活多胺合成通路调控病毒侵染的分子机理，揭示RSMV基质蛋白M与OAZ互作在病毒粒体装配中功能，解析OAZ、ODC和多胺在介体昆虫持久传播RSMV过程中真正扮演的角色；并比较分析水稻寄主多胺合成通路参与RSMV侵染的情况。研究结果将为探索从多胺通路阻断RSMV侵染的有效策略提供重要的理论依据。

Rice stripe mosaic virus (RSMV) is a newly identified rice cytorhabdovirus transmitted by the leafhopper Recilia dorsalis. We previously found that the production of polyamines, as well as the key gene ornithine decarboxylase antizyme (OAZ) associated with polyamine synthesis pathway, was significantly induced in RSMV infected insects and plants, and OAZ specifically interacted with RSMV M protein. We deduced that RSMV may manipulate polyamine synthesis in insect, thereby favouring its propagation. This project intends to take the RSMV transmitted by the vector Recilia dorsalis as the main research object, and combines cell biology, electron microscopy, immunofluorescence labeling, RNA interference and other molecular techniques to reveal the mechanism for the activation of insect polyamine synthesis pathway by Rice stripe mosaic virus to promote viral infection. The study will unravel the molecular mechanism of interaction between OAZ and M protein involved in RSMV infection, and analyze the roles of polyamines and the key biosynthetic enzymes of vector insects in the process of persistent transmission of RSMV. In addition, we will analyze whether the polyamines pathway of plant is beneficial to the infection of RSMV. These studies will provide valuable clues for designing new strategies to block RSMV infection from polyamine pathway in vectors and hosts.