艳山姜调控HMGB1-NF-κB级联通路逆转血管内皮间质转化机制研究

前期研究发现，艳山姜挥发油具有保护血管内皮作用以及抑制血管重构的作用，而血管内皮间质转化（Endo-MT）是介导内皮功能障碍与血管重构的关键病理环节。本研究提出基于调控关键通路HMGB1-NF-κB可能是艳山姜逆转内皮间质转化的关键分子机制假说。研究应用TGF-β建立Endo-MT细胞损伤模型，建立自发性高血压大鼠血管Endo-MT动物模型，采用GC-MS等分析手段，运用细胞电镜、免疫组化等进行形态学分析，相关生化酶学及PCR、Western blot等现代分子生物学技术，系统分析艳山姜挥发油逆转血管内皮间质转化作用，进一步揭示其靶向保护血管内皮的作用机制。本研究为艳山姜民族特色药物资源的挖掘与开发提供科学依据，为民族医药防治心血管疾病提供理论基础，指导临床。

Based on the preliminary discovery that Alpinia zerumbet regulates vascular endothelial function and endothelium-dependent correlation, focusing on the key point in Endothelial-to-mesenchymal transition （Endo-MT）, we propose the hypothesis that ethnicmedicine Alpinia zerumbet inhibits Endo-MT through the regulation of HMGB1-NF-κB signal pathway. In this study, we applied TGF-β-induced Endo-MT cell injury model, and built endothelial injury in spontaneously hypertensive rat animal model. Utilizing GC-MS fingerprints and other modern analytical methods, along with cell electron microscopy morphological analysis, and modern molecular biology techniquessuch as biochemical enzymatic and PCR, western blot, we systemmatically analyzed essential oil from Alpinia zerumbet’s function of reversing Endo-MT and its protective effect vascular endothelial function. This study provides insights and scientific basis to the use and further systematical research and development of Alpinia zerumbet resource, which promotes the advance of ethnic medicine on treating cardiovascular diseases, and also provides guidance to the clinic trials.