热带禾本科与豆科牧草表面附着乳酸菌多样性差异及其对青贮发酵影响研究

牧草表面附着乳酸菌的种类和数量对青贮发酵有着重要影响，本项目以主要优良热带牧草柱花草、王草为研究对象，首先通过高通量测序技术、16SrDNA序列分析传统分离培养法相结合和实时荧光定量PCR对不同牧草、不同生长期和刈割时间表面及其青贮中乳酸菌的种类和数量进行研究，分析其多样性和变化规律；其次，从新鲜牧草和青贮中筛选出优良乳酸菌菌株，研究其对不同种属热带牧草青贮发酵的影响；该研究成果将为热带牧草开发利用乳酸菌制剂调制青贮饲料提供科学的理论依据。

The epiphytic lactic acid bacteria that are present on the plants play an important role in silage fermentation processes. In this research, taking high quality forage of Stylosanthes guianensis and Pennisetum purpureum × P. americana as research materials, high throughput sequencing technology in combined with traditional culture dependent methods ,sequence analysis of 16SrDNA and real time qPCR were used to investigate the diversity,species and numbers of lactic acid bacteria communities during the growth and ensiling. Further ,excellent epiphytic lactic acid bacteria were identified and isolated from fresh grass and silages and their influence on silage fermentation were evaluated. The research results will provide important information for development and utilization of inoculants to make good quality silages in tropical area.