番茄茉莉酸信号途径抑制子基因SlJAZ2/9/10/11在病原菌防御中的功能及调控机理研究

番茄是一种研究果实发育及成熟的模式植物，也是重要的蔬菜作物，番茄种植过程中病害的发生严重威胁番茄产业发展。番茄抗病育种成为防治病害最彻底、有效、环保的手段。茉莉酸在植物生物胁迫特别是病原菌胁迫响应中发挥重要的调控作用。申请者前期获得了茉莉酸信号途径抑制子基因SlJAZ2/10/11的超表达转基因番茄植株，并对SlJAZ2和SlJAZ11超表达转基因番茄表型进行了初步分析，发现SlJAZ2可能在生物胁迫响应中发挥重要作用。本研究拟在此基础上，通过对SlJAZ2/9/10/11基因敲除和超表达转基因番茄对丁香假单胞菌的抗病性分析、SlJAZs调控转录因子分析、互作蛋白筛选与验证等实验，对SlJAZs基因功能进行研究，明确SlJAZs的调控途径，结果有助于完善茉莉酸参与的病原菌胁迫响应调控网络，为番茄及其它茄科植物的抗病育种提供理理论基和基因资源。

Tomato is not only a model plant for fruit development and ripening studying, but also an important vegetable crop. The diseases occurring in tomato planting seriously threaten the development of tomato industry. Tomato disease-resistant breeding is becoming the most thorough, effective and environmentally friendly mean of disease control. Jasmonic acid plays an important regulatory role in response to plant biotic stresses, especially the pathogenic bacteria infection. We obtained the overexpression transgenic tomato plants of Jasmonic acid signal pathway inhibitor genes SlJAZ2/10/11 in earlier study, and analyzed the phenotypes of SlJAZ2 and SlJAZ11. We found that SlJAZ2 may play important role in biotic stress response. Our research intends to study the function of SlJAZs gene and identify the regulatory pathway of SlJAZs by analyzing the resistibility of SlJAZ2/9/10/11 knockout and overexpression transgenic tomato to Pseudomonas syringae pv. tomato, finding out the genes regulated by SlJAZs, screening and validating of interaction proteins and so on. These results will help to improve the regulatory network of jasmonic acid-mediated pathogen response and provide theoretical basis and genetic resources for disease-resistant breeding of tomato and other Solanaceous plants.