大豆miRNA重复基因与靶基因的共进化分析

大豆是重要的粮食作物，目前的大豆危机已严重威胁了我国粮食安全。大豆重要功能基因的挖掘利用对于大豆生产具有重要意义，miRNA亦是一类重要的基因调控元件。大豆基因组在最近的60万年间发生了两次大的重复事件，多倍化后重复编码基因对和重复miRNA基因对在功能上都发生了不同程度的分化，然而关于miRNA重复基因对的功能分化是否会改变其对靶基因的调控、miRNA重复基因对的功能分化与其对应靶基因的功能分化之间是否存在共进化关系等还未见报道。本课题在前期研究的基础上，拟通过转录组、降解组等手段，找出miRNA重复基因对所对应靶基因以及它们之间的异同，利用生物信息学和分子生物学方法系统解析影响大豆重复miRNA基因对表达分化的因素和重复miRNA基因对功能分化后对靶基因的影响，从而揭示大豆中重复miRNA基因和靶基因的共进化规律，为深入理解miRNA在大豆基因组进化中的作用提供实验证据和理论基础。

Soybean (Glycine max L. Merr.) is the most important crops in China. The crisis of soybean have serious threat to the food security of our country. Identify and utility soybean important functional genes will benefit for soybean industry. miRNA is one of most important element of gene regulation. Soybean genome has putatively processed two rounds of whole genome duplications (WGD) and many tandem duplications within the last 60 million years. It has been suggested that duplicated coding gene pairs are highly diversified after numerous chromosome rearrangements. However, the evolution of miRNA, such as whether duplicated pairs also existed and if their functions had been diversified as coding genes, and the co-evolution of them with their target genes are poorly understood. On the base of our previous study, we are trying to do a comprehensive analysis on the duplicated miRNAs genes through transcriptomics and degradomics approaches to find out the factors that influencing duplicated miRNA gene evolution and their co-evolution with target genes. The findings will shed light on the role of miRNA in the evolution of polyploide soybean genome. In addition, it may also benefit the soybean functional genome analysis.