玉米ZmPAT1基因调控根系响应低磷胁迫的分子机制

鉴定作物应答低磷胁迫的关键基因，是开展磷高效品种选育的重要基础。项目组依据前期对低磷胁迫下玉米苗期根系性状的全基因组SNP标记关联分析等结果，发掘了一个含有拓扑异构酶II相关蛋白结构域的新基因ZmPAT1，经连锁分析和分子生物学实验分析，初步证实了该基因具有响应低磷胁迫并与根系及产量性状相关，并检测到该基因蛋白与磷信号通路的关键基因SPX蛋白可以互作。因此，本项目将采用候选基因关联分析、连锁分析和分子生物学相结合的方法，研究目标基因的生物学功能及其调控根系响应低磷胁迫的分子机制；同时，从优异种质中发掘该基因的优良等位变异，为玉米磷高效利用性状的遗传改良，提供理论和技术参考。

Study on genetic regulation in root adaption under low-phosphorus (P) stress is the key basis for crop improvement with high P efficiency. In this proposal, a novel gene ZmPAT1 which contains an topoisomerase II domain (PAT) has been screened and identified based on the previous project results of genome-wide association study by SNPs and genome-wide expression profile of maize seedling root traits under P starvation at early stage. The candidate gene association analysis and quantitative expression experiments preliminarily uncovered this gene responses to low-P stress and possesses genetic effect in regulation of root and yield. Accordingly, our project will combine candidate gene association analysis, linkage analysis and molecular biology to study the biological function and molecular merchanism of regulation in root to response low-P stress of target gene, as well as provide theoretical and technical reference for genetic improvement of maize high P-use efficiency traits by mining excellent allelic variations of this gene from elite germplasms.